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Prepared by the eResearch Office, QUT.

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Code Block
  nextflow run nf-core/rnaseq \
      --input samplesheet.csv \
      --genome GRCm38 \
      --aligner star \
      -profile condasingularity

Note, if the running was interrupted or did not complete a particular step or you want to modify a parameter for a particular step, instead of re-running all process again nextflow enables to “-resume” the workflow.

Code Block
  nextflow run nf-core/rnaseq \
      --input samplesheet.csv \
      --genome GRCm38 \
      --aligner star \
      -profile singularity conda\
      -resume

Preparing a ‘samplesheet.csv’ file

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In the folder you have created for this run create launch.pbs using a text editor (i.e., vim, nano)

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