...
| Code Block |
|---|
nextflow run nf-core/rnaseq --input samplesheet.csv \
--outdir results \
-r 3.10.1 \
--genome GRCh38 \
-profile singularity \
--aligner star_rsem \
--clip_r1 10 \
--clip_r2 10 \
--three_prime_clip_r1 12 \
--three_prime_clip_r2 12 |
Note, if the running was interrupted or you did not complete a particular step, or you want to modify a parameter for a particular step, instead of re-running all processes again, nextflow enables you to “-resume” the workflow.
| Code Block |
|---|
nextflow run nf-core/rnaseq --input samplesheet.csv \
--outdir results \
-r 3.10.1 \
--genome GRCh38 \
-profile singularity \
--aligner star_rsem \
--clip_r1 10 \
--clip_r2 10 \
--three_prime_clip_r1 12 \
--three_prime_clip_r2 12 \
-resume |
Preparing a ‘samplesheet.csv’ file
...