Pre-requisites
Run your samples (FASQFASTQ) using the nextflow nf-core/RNA-seq pipeline using ‘star_salmon’ or an alternative pipeline that generates feature counts. For example:
Code Block xaccession gene M.209.3D.C M.647.C M.711.C M.8.C M.996.C M.996.T M.209.3D.T M.647.T M.711.T M.8.T NM_000014.4 A2M 78132 51570 32120 73355 106630 111500 93300 51695 106295 120380 NM_000015.2 NAT2 0 0 0 0 0 0 2 0 0 0 NM_000016.5 ACADM 1241 1614 1436 1192 2962 2127 1579 1471 2084 1123 NM_000017.2 ACADS 799 987 984 1010 2407 1460 1050 879 1462 816 NM_000018.3 ACADVL 6025 5078 4570 3725 8332 8693 8144 6549 10236 6501 NM_000019.3 ACAT1 1020 1178 1272 1109 2781 1400 1266 1011 1575 824 NM_000020.2 ACVRL1 0 51 137 0 132 220 0 237 0 22 NM_000021.3 PSEN1 367 911 355 766 1469 1287 1201 1248 1400 994 NM_000022.2 ADA 77 177 124 62 261 234 104 141 232 58 NM_000023.2 SGCA 0 0 0 0 0 0 0 0 0 0 NM_000024.5 ADRB2 671 1002 336 171 426 166 692 847 277 79 NM_000025.2 ADRB3 0 0 0 0 0 0 1 0 0 0 NM_000026.2 ADSL 1243 1597 1214 1441 2420 1842 1665 1424 1964 1281 NM_000027.3 AGA 535 712 412 358 1104 1017 727 716 915 391
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