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Code Block
#!/bin/bash -l
#PBS -N nfsmrnaseq
#PBS -l select=1:ncpus=2:mem=4gb
#PBS -l walltime=24:00:00

cd $PBS_O_WORKDIR
module load java
NXF_OPTS='-Xms1g -Xmx4g'

nextflow run nf-core/smrnaseq -r 2.1.0 \
	-profile singularity \
	--outdir outdir \
	--input samplesheet.csv \
	--genome GRCh38 \
	--three_prime_adapter 'AACTGTAGGCACCATCAAT'\
	--fastp_min_length 18 \
	--fastp_max_length 30 \
	--hairpin /work/trtp/data/mirbase/hairpin.fa \
	--mature /work/trtp/data/mirbase/mature.fa

Submit the job to the HPC cluster:

Code Block
qsub launch_phase3.pbs

monitor Monitor the progress on the HPC:

Code Block
qjobs

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