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wget https://www.mirbase.org/download/CURRENT/hairpin.fa gzip -c hairpin.fa > hairpin.fa.gz_file/mature.fa |
Hairpin sequences:
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wget https://www.mirbase.org/download_file/CURRENT/maturehairpin.fa gzip -c mature.fa.gz |
Alternatively, submit the following PBS Pro script to the cluster. Before running the script, create a ‘reference’ folder (i.e., /myteam/data/reference/ ).
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#!/bin/bash -l #PBS -N nfsmrnaseq #PBS -l select=1:ncpus=2:mem=4gb #PBS -l walltime=24:00:00 cd $PBS_O_WORKDIR wget https://www.mirbase.org/download/CURRENT/hairpin.fa gzip -c hairpin.fa > _file/hairpin.fa.gz wget https://www.mirbase.org/download_file/CURRENT/mature.fa gzip -c mature.fa.gz |
Run a test
Before running the pipeline with real data, run the following test:
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