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A HPC account. If you do not have one, please request one here.
Access to an rVDI virtual desktop machine with 64GB RAM. For information about rVDI and requesting a virtual machine, see here.
Nextflow installed on your HPC home account. If you haven’t already installed Nextflow, do so by following the guide here.
Your scRNA-Seq data (fastq files) are on the HPC. If you are having difficulties transferring them to the HPC, submit a support ticket here.
Connect to an rVDI virtual desktop machine
scRNA-Seq datasets are often very large, requiring a lot of memory to run. Downstream analysis is run in R, on a Windows machine. Your PC is unlikely to have enough RAM, thus we’re using virtual machines. In addition, you can run the rest of the analysis in the virtual machine.
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