Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

Data

Experiment Accession

sample

FASTQ

Experiment Title

Organism Name

Instrument

Submitter

Study Accession

Study Title

Sample Accession

Total Size, Mb

Total Spots

Total Bases

Library Strategy

Library Source

Library Selection

SRX14748451

S1

SRR18645307

Homo sapiens

Homo sapiens

MinION

Drexel University

SRP367676

Multiplex structural variant detection by whole-genome mapping and nanopore sequencing.

SRS12509856

821.1

348226

972620520

OTHER

GENOMIC

other

SRX19406878

S2

SRR23513621

NA12878 DNA sequencing from nanopore WSG consortium - basecalled sequences (Guppy 6.1.3 super accuracy)

Homo sapiens

MinION

Garvan Institute of Medical Research

SRP421403

Curated publicly available nanopore datasets

SRS16801715

78526.8

11173458

97545895593

WGS

GENOMIC

RANDOM

SRX14748451

ERX8211413

Homo sapiens

S3

Homo sapiens

ERR8578833

MinION

Drexel University

SRP367676

Multiplex structural variant detection by whole-genome mapping and nanopore

sequencing

.

SRS12509856

821.1

348226

972620520

ERX8211414

MinION sequencing

OTHER

GENOMIC

other

Homo sapiens

MinION

the university of hong kong

ERP135493

Target enrichment sequencing and variant calling on medical exome using ONT MinION

ERS10590135

10669

8961.

72

02

10644000

9636172

12212807287

10382057986

Targeted-Capture

GENOMIC

PCR

ERX8211414

S4

ERX8211413

ERR8578834

MinION sequencing

Homo sapiens

MinION

the university of hong kong

ERP135493

Target enrichment sequencing and variant calling on medical exome using ONT MinION

ERS10590135

8961

10669.

02

72

9636172

10644000

10382057986

12212807287

Targeted-Capture

GENOMIC

PCR

SRX13322984

S5

SRX13323057

SRR17138639

Nanopore targeted sequencing (ReadUntil/ReadFish) of NA12878-HG001- basecalled sequences

Homo sapiens

MinION

Garvan Institute of Medical Research

SRP349335

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted nanopore sequencing

SRS11230747

SRS11230712

17107

6629.

98

97

12278391

5513156

20238395479

7815960904

WGS

GENOMIC

other

SRX13323057

S6

SRX13322984

SRR17138566

Nanopore targeted sequencing (ReadUntil/ReadFish) of NA12878-HG001- basecalled sequences

Homo sapiens

MinION

Garvan Institute of Medical Research

SRP349335

Comprehensive genetic diagnosis of tandem repeat expansion disorders with programmable targeted nanopore sequencing

SRS11230712

SRS11230747

6629

17107.

97

98

5513156

12278391

7815960904

20238395479

WGS

GENOMIC

other

Mapping

Let’s run the --help option of the pipeline to get information on the available parameters

Code Block
module load java
nextflow run epi2me-labs/wf-alignment -profile singularity --help
Code Block
N E X T F L O W  ~  version 23.12.0-edge
Launching `https://github.com/epi2me-labs/wf-alignment` [nostalgic_galileo] DSL2 - revision: e1fd7a51dc [master]
WARN: Config setting `prov.formats` is not defined, no provenance reports will be produced

||||||||||   _____ ____ ___ ____  __  __ _____      _       _
||||||||||  | ____|  _ \_ _|___ \|  \/  | ____|    | | __ _| |__  ___
|||||       |  _| | |_) | |  __) | |\/| |  _| _____| |/ _` | '_ \/ __|
|||||       | |___|  __/| | / __/| |  | | |__|_____| | (_| | |_) \__ \
||||||||||  |_____|_|  |___|_____|_|  |_|_____|    |_|\__,_|_.__/|___/
||||||||||  wf-alignment v1.1.2-ge1fd7a5
--------------------------------------------------------------------------------
Typical pipeline command:

  nextflow run epi2me-labs/wf-alignment \ 
        --fastq 'wf-alignment-demo/fastq' \ 
        --references 'wf-alignment-demo/references'

Input Options
  --fastq                [string]  FASTQ files to use in the analysis.
  --bam                  [string]  BAM or unaligned BAM (uBAM) files to use in the analysis.
  --analyse_unclassified [boolean] Analyse unclassified reads from input directory. By default the workflow will not process reads in the unclassified 
                                   directory. 
  --references           [string]  Path to a directory containing FASTA reference files.
  --reference_mmi_file   [string]  Path to an MMI index file to be used as reference.
  --counts               [string]  Path to a CSV file containing expected counts as a control.

Sample Options
  --sample_sheet         [string]  A CSV file used to map barcodes to sample aliases. The sample sheet can be provided when the input data is a directory 
                                   containing sub-directories with FASTQ files. 
  --sample               [string]  A single sample name for non-multiplexed data. Permissible if passing a single .fastq(.gz) file or directory of .fastq(.gz) 
                                   files. 

Output Options
  --out_dir              [string]  Directory for output of all workflow results. [default: output]
  --prefix               [string]  Optional prefix attached to each of the output filenames.

Advanced options
  --depth_coverage       [boolean] Calculate depth coverage statistics and include them in the report. [default: true]
  --minimap_preset       [choice]  Pre-defined parameter sets for `minimap2`, covering most common use cases. [default: dna]
                                   * dna
                                   * rna
  --minimap_args         [string]  String of command line arguments to be passed on to `minimap2`.

Miscellaneous Options
  --threads              [integer] Number of CPU threads to use for the alignment step. [default: 4]
  --disable_ping         [boolean] Enable to prevent sending a workflow ping.

Other parameters
  --monochrome_logs      [boolean] null
  --validate_params      [boolean] null [default: true]
  --show_hidden_params   [boolean] null

!! Hiding 4 params, use --show_hidden_params to show them !!
--------------------------------------------------------------------------------
If you use epi2me-labs/wf-alignment for your analysis please cite:

* The nf-core framework
  https://doi.org/10.1038/s41587-020-0439-x