Versions Compared

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

Create working folder and copy data

...

Create a conda environment called ONTvariants_nanoplotQC

Code Block
conda create -n ONTvariants_QC
Code Block
Collecting package metadata (current_repodata.json): done
Solving environment: done


## Package Plan ##

  environment location: /home/barrero/miniconda3/envs/ONTvariants_nanoplotQC


Proceed ([y]/n)? y

Preparing transaction: done
Verifying transaction: done
Executing transaction: done
#
# To activate this environment, use
#
#     $ conda activate ONTvariants_nanoplotQC
#
# To deactivate an active environment, use
#
#     $ conda deactivate

...

As outputs find the porechop_abi processed file (SRR17138639_1_porechop_abi.fastq) and the chopper output (SRR17138639_1_porechop_abi_chopper_q10_300b.fastq). To visualise the QC reports, let’s connect to the HPC via file finder (see below).

NOTE: To proceed, you need to be on QUT’s WiFi network or signed via VPN.

To browse the working folder in the HPC type in the file finder:

...