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In session 2 and 3 (variant calling) we submitted jobs to the HPC via a PBS script. This is useful for large datasets that require lots of processing time or resources. For smaller datasets (like 16S amplicon sequence data), you can start ‘interactive mode’ on the HPC, which allocates you a temporary node with RAM/CPUs you request.

Open PuTTy and paste the text below into the command prompt:

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