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In the ‘2.Setup’ section, you downloaded 3 nfcore/sarek runs to H:/workshop/sarek/runs. These represent sarek variant calling results from the 3 exercises run in session 2:

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SNPeff creates its own variant analysis reports (html reports) for each sample, which can be seen here:

H:/workshop/sarek/runs/run2_trio/results/reports/snpeff/haplotypecaller

We’ll be creating our of reports, with some additional functional information, using R.

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Click ‘Knit’ to generate the report. It will ask you to name the file and ask where to save the file. Call it ‘test’ and save it in H:/workshop

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You will see it generates the test html report.

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In RStudio, select ‘File’ → “Open File”

Browse to H:/workshop/sarek/runs/run2run3_trioliver

Select the ‘modify.R’ file and click ‘Open’.

Open the ‘function_report.Rmd’ file in the same way (it’s in the same location as modify.R)

These are the two files you’ll need to generate the reports.G:\Other computers\Home PC\Desktop\QUT\Liver_exome_variants\sarek