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Overview

  • Similar to exercise 6.4 we will:

    • Use created “samplesheet.csv” metadata file for small RNAseq datasets in exercise 6.4.

    • Use a “nextflow.config” file in the working directory to override Nextflow parameters (e.g., specify where to find the pipeline assets).

    • Use a PBS script to run the expression profiling of miRNAs against MirGeneDB, a curated database that includes experimentally validated miRNAs.

Prepare pipeline inputs

Let’s move to the working directory:

...

Code Block
cat mature_counts.csv
Code Block
"","hsaHsa-letLet-7a7-P1b_5p","hsaHsa-letLet-7a7-3pP1c_5p","hsa-let-7a-2-3pHsa-Let-7-P1d_5p","Hsa-Let-7-P2a1_5p","hsaHsa-letLet-7b7-P2a2_5p","hsaHsa-letLet-7b7-3pP2a3_5p","hsaHsa-letLet-7c7-P2b1_5p","hsaHsa-letLet-7c7-3pP2b2_5p","hsaHsa-letLet-7d7-P2b3_5p","hsaHsa-letLet-7d7-3pP2c1_5p","hsa-
"ERR409882",364608,341,16,59417,1998,68342,44,14861,3790,29486,207,211184,228,1462,7002,2,49664,1,1091,174,326,43,6,468,7,1482,1615,9,17256,534,573,6526,0
"ERR409879",305651,184,6,52115,1476,58425,30,12397,2659,23604,201,198778,151,1013,5486,1,48381,4,945,202,194,40,7,368,3,1097,1317,6,12662,561,372,3693,2,1
"ERR409881",712880,165,9,83857,2335,162724,83,30556,4503,68044,385,456864,348,1818,9893,0,111712,5,1495,259,174,48,6,318,2,1466,2220,4,17865,466,551,10360
"ERR409884",182178,111,3,27892,913,39989,21,7751,1886,13902,159,127386,132,743,3651,3,40311,0,629,117,97,21,11,305,2,1147,902,2,8313,368,242,2276,0,1146,4
"ERR409889",568269,257,13,92339,2239,100021,45,20819,3511,44172,207,276474,259,1376,12407,5,83908,5,1971,467,403,70,30,1082,7,3082,3172,14,24112,819,421,6
"ERR409894",314053,137,9,44708,1220,74145,74,12313,2827,25295,196,196866,158,896,4681,3,43677,1,806,138,131,22,7,296,3,1181,1169,5,11145,611,360,3742,5,12
"ERR409887",178201,48,4,25678,733,41506,27,7833,1613,15724,121,123391,98,497,3288,0,39434,1,445,97,65,15,3,150,2,539,461,3,5837,186,161,2958,2,847,3,1544,
"ERR409880",318121,136,3,46347,1260,65606,39,11095,2269,24585,200,191072,194,1118,5599,2,67420,3,1242,155,168,22,2,505,6,1708,1836,3,11293,482,359,3652,1,
"ERR409890",332579,105,7,40131,955,73537,38,13528,2029,31807,158,207846,175,962,5146,0,42402,0,659,149,102,20,4,219,3,964,1086,4,11957,423,385,6017,4,1556Hsa-Let-7-P2c2_5p","Hsa-Let-7-P2c3_5p","Hsa-Mir-1-P1_3p","Hsa-Mir-1-P2_3p","Hsa-Mir-1-P3_3p","Hsa-Mir-7-P1_5p","Hsa-Mir-7-P2_5p","Hsa-Mir-7-P4_5p","Hsa-Mir-8-P1a_3p","Hsa-Mir-8-P1b_3p","Hsa-Mir-8-P2a_3p","Hsa-Mir-8-P2b_3p","Hsa-Mir-8-P3a_3p","Hsa-Mir-9-P1_5p","Hsa-Mir-9-P1_3p","Hsa-Mir-9-P2_5p","Hsa-Mir-9-P2_3p","Hsa-Mir-9-P3_5p","Hsa-Mir-9-P3_3p","Hsa-Mir-10-P1b-v1_5p","Hsa-Mir-10-P1b-v2_5p","Hsa-Mir-10-P1c-v1_5p","Hsa-Mir-10-P1c-v2_5p","Hsa-Mir-10-P2b_5p","Hsa-Mir-10-P2c_5p","Hsa-Mir-10-P2d_5p","Hsa-Mir-10-P3b_5p","Hsa-Mir-10-P3c_5p","Hsa-Mir-10-P3d_5p","Hsa-Mir-15-P1a_5p","Hsa-Mir-15-P1b_5p","Hsa-Mir-15-P1c_5p","Hsa-Mir-15-P1d_5p","Hsa-Mir-15-P2a_5p","Hsa-Mir-15-P2b_5p","Hsa-Mir-15-P2c_5p","Hsa-Mir-15-P2d_5p","Hsa-Mir-17-P1a_5p","Hsa-Mir-17-P1c_5p","Hsa-Mir-17-P1d_5p","Hsa-Mir-17-P1d_3p","Hsa-Mir-17-P2a_5p","Hsa-Mir-17-P2c_5p","Hsa-Mir-17-P4a_5p","Hsa-Mir-17-P4c_5p","Hsa-Mir-17-P4d_5p","Hsa-Mir-19-P1a_3p","Hsa-Mir-19-P2a_3p","Hsa-Mir-19-P2c_3p","Hsa-Mir-21_5p","Hsa-Mir-22-P1b_3p","Hsa-Mir-23-P2_3p","Hsa-Mir-23-P3_3p","Hsa-Mir-24-P2_3p","Hsa-Mir-24-P3_3p","Hsa-Mir-26-P1_5p","Hsa-Mir-26-P2_5p","Hsa-Mir-26-P4_5p","Hsa-Mir-27-P2_3p","Hsa-Mir-27-P3_3p","Hsa-Mir-28-P1_5p","Hsa-Mir-28-P1_3p","Hsa-Mir-28-P2_5p","Hsa-Mir-28-P2_3p","Hsa-Mir-28-P3_5p","Hsa-Mir-29-P1b-v1_3p","Hsa-Mir-29-P1b-v2_3p","Hsa-Mir-29-P1d-v1_3p","Hsa-Mir-29-P1d-v2_3p","Hsa-Mir-29-P2b_3p","Hsa-Mir-29-P2d_3p","Hsa-Mir-30-P1a_5p","Hsa-Mir-30-P1b_5p","Hsa-Mir-30-P1d_5p","Hsa-Mir-30-P2a_5p","Hsa-Mir-30-P2b_5p","Hsa-Mir-30-P2d_5p","Hsa-Mir-31_5p","Hsa-Mir-32_5p","Hsa-Mir-33-P1_5p","Hsa-Mir-33-P3_5p","Hsa-Mir-34-P1_5p","Hsa-Mir-34-P2a_5p","Hsa-Mir-34-P2a_3p","Hsa-Mir-34-P2b_5p","Hsa-Mir-34-P3a-v1_5p","Hsa-Mir-34-P3a-v2_5p","Hsa-Mir-34-P3b_5p","Hsa-Mir-34-P3b_3p","Hsa-Mir-34-P3c_5p","Hsa-Mir-92-P1a_3p","Hsa-Mir-92-P1c_3p","Hsa-Mir-92-P1d_3p","Hsa-Mir-92-P2a_3p","Hsa-Mir-92-P2c_3p","Hsa-Mir-92-P2d_3p","Hsa-Mir-95-P1_3p","Hsa-Mir-95-P2_3p","Hsa-Mir-95-P3-v1_5p","Hsa-Mir-95-P3-v2_5p","Hsa-Mir-95-P4_5p","Hsa-Mir-95-P4_3p","Hsa-Mir-96-P1_5p","Hsa-Mir-96-P2_5p","Hsa-Mir-96-P3-v1_5p","Hsa-Mir-96-P3-v2_5p","Hsa-Mir-101-P1-v1_3p","Hsa-Mir-101-P1-v2_3p","Hsa-Mir-101-P2-v1_3p","Hsa-Mir-101-P2-v2_3p","Hsa-Mir-103-P1_3p","Hsa-Mir-103-P2_3p","Hsa-Mir-103-P4_3p","Hsa-Mir-105-P1_5p","Hsa-Mir-105-P2_5p","Hsa-Mir-122_5p","Hsa-Mir-124-P1-v1_3p","Hsa-Mir-124-P1-v2_3p","Hsa-Mir-124-P2-v1_3p","Hsa-Mir-124-P2-v2_3p","Hsa-Mir-124-P3-v1_3p","Hsa-Mir-124-P3-v2_3p","Hsa-Mir-126-P2-v1_3p","Hsa-Mir-126-P2-v2_5p","Hsa-Mir-126-P2-v3_3p","Hsa-Mir-127_3p","Hsa-Mir-128-P1_3p","Hsa-Mir-128-P2_3p","Hsa-Mir-129-P1_3p","Hsa-Mir-129-P2_5p","Hsa-Mir-129-P2_3p","Hsa-Mir-130-P1c_3p","Hsa-Mir-130-P2a_3p","Hsa-Mir-130-P2b_3p","Hsa-Mir-130-P3b_3p","Hsa-Mir-130-P4a_5p","Hsa-Mir-130-P4a_3p","Hsa-Mir-132-P1_3p","Hsa-Mir-132-P2_5p","Hsa-Mir-132-P2_3p","Hsa-Mir-133-P1-v1_3p","Hsa-Mir-133-P1-v2_3p","Hsa-Mir-133-P2-v1_3p","Hsa-Mir-133-P2-v2_3p","Hsa-Mir-133-P3-v1_3p","Hsa-Mir-133-P3-v2_3p","Hsa-Mir-134_5p","Hsa-Mir-135-P1_5p","Hsa-Mir-135-P2_5p","Hsa-Mir-135-P4_5p","Hsa-Mir-136-v1_3p","Hsa-Mir-136-v2_5p","Hsa-Mir-136-v2_3p","Hsa-Mir-137-P1-v1_3p","Hsa-Mir-137-P1-v2_3p","Hsa-Mir-138-P1_5p","Hsa-Mir-138-P2_5p","Hsa-Mir-139_5p","Hsa-Mir-140-P1-v1_3p","Hsa-Mir-140-P1-v2_3p","Hsa-Mir-142-P1-v1_5p","Hsa-Mir-142-P1-v2_3p","Hsa-Mir-142-P1-v3_3p","Hsa-Mir-142-P1-v4_5p","Hsa-Mir-143_3p","Hsa-Mir-144-v1_3p","Hsa-Mir-144-v2_3p","Hsa-Mir-145_5p","Hsa-Mir-146-P1_5p","Hsa-Mir-146-P4_5p","Hsa-Mir-147-v1_3p","Hsa-Mir-147-v2_3p","Hsa-Mir-148-P1_3p","Hsa-Mir-148-P3_3p","Hsa-Mir-148-P4_3p","Hsa-Mir-149_5p","Hsa-Mir-150_5p","Hsa-Mir-153-P1_3p","Hsa-Mir-153-P2_3p","Hsa-Mir-154-P1_5p","Hsa-Mir-154-P1_3p","Hsa-Mir-154-P2-v1_5p","Hsa-Mir-154-P2-v2_5p","Hsa-Mir-154-P3a-v1_3p","Hsa-Mir-154-P3a-v2_3p","Hsa-Mir-154-P3b_3p","Hsa-Mir-154-P4a_3p","Hsa-Mir-154-P4b_3p","Hsa-Mir-154-P5_5p","Hsa-Mir-154-P5_3p","Hsa-Mir-154-P6_3p","Hsa-Mir-154-P7_5p","Hsa-Mir-154-P8_5p","Hsa-Mir-154-P8_3p","Hsa-Mir-154-P9_3p","Hsa-Mir-154-P10_5p","Hsa-Mir-154-P12_3p","Hsa-Mir-154-P13-v1_5p","Hsa-Mir-154-P13-v2_5p","Hsa-Mir-154-P14_5p","Hsa-Mir-154-P15_5p","Hsa-Mir-154-P16_3p","Hsa-Mir-154-P17_3p","Hsa-Mir-154-P18_3p","Hsa-Mir-154-P19_3p","Hsa-Mir-154-P20-v1_3p","Hsa-Mir-154-P20-v2_3p","Hsa-Mir-154-P21_3p","Hsa-Mir-154-P22_3p","Hsa-Mir-154-P23_3p","Hsa-Mir-154-P24_3p","Hsa-Mir-154-P25_3p","Hsa-Mir-154-P26_3p","Hsa-Mir-154-P27_3p","Hsa-Mir-154-P28a_3p","Hsa-Mir-154-P28b_5p","Hsa-Mir-154-P28b_3p","Hsa-Mir-154-P29_3p","Hsa-Mir-154-P30_3p","Hsa-Mir-154-P36_3p","Hsa-Mir-155_5p","Hsa-Mir-181-P1a_5p","Hsa-Mir-181-P1b_5p","Hsa-Mir-181-P1c_5p","Hsa-Mir-181-P2a_5p","Hsa-Mir-181-P2b_5p","Hsa-Mir-181-P2c_5p","Hsa-Mir-184_3p","Hsa-Mir-185_5p","Hsa-Mir-186_5p","Hsa-Mir-187_3p","Hsa-Mir-188-P1_5p","Hsa-Mir-188-P2_5p","Hsa-Mir-188-P3_5p","Hsa-Mir-190-P1_5p","Hsa-Mir-190-P3_5p","Hsa-Mir-191_5p","Hsa-Mir-192-P1_5p","Hsa-Mir-192-P2_5p","Hsa-Mir-193-P1a_3p","Hsa-Mir-193-P1b_5p","Hsa-Mir-193-P2a_3p","Hsa-Mir-193-P2b_3p","Hsa-Mir-194-P1_5p","Hsa-Mir-194-P2_5p","Hsa-Mir-196-P1_5p","Hsa-Mir-196-P3_5p","Hsa-Mir-196-P4_5p","Hsa-Mir-197_3p","Hsa-Mir-199-P1-v1_3p","Hsa-Mir-199-P1-v2_3p","Hsa-Mir-199-P1-v3_3p","Hsa-Mir-199-P2-v1_3p","Hsa-Mir-199-P2-v2_3p","Hsa-Mir-199-P2-v3_3p","Hsa-Mir-199-P3-v1_3p","Hsa-Mir-199-P3-v2_3p","Hsa-Mir-199-P3-v3_3p","Hsa-Mir-202_5p","Hsa-Mir-203-v1_3p","Hsa-Mir-203-v2_3p","Hsa-Mir-204-P1_5p","Hsa-Mir-204-P2_5p","Hsa-Mir-205-P4_5p","Hsa-Mir-208-P1_3p","Hsa-Mir-208-P2_3p","Hsa-Mir-210_3p","Hsa-Mir-214-v1_3p","Hsa-Mir-214-v2_3p","Hsa-Mir-216-P2a_5p","Hsa-Mir-216-P2b_5p","Hsa-Mir-217-v1_5p","Hsa-Mir-217-v2_5p","Hsa-Mir-218-P2_5p","Hsa-Mir-218-P4_5p","Hsa-Mir-219-P2_5p","Hsa-Mir-219-P2_3p","Hsa-Mir-219-P2-as_5p","Hsa-Mir-219-P2-as_3p","Hsa-Mir-219-P3_3p","Hsa-Mir-221-P1a_3p","Hsa-Mir-221-P2a_5p","Hsa-Mir-221-P2a_3p","Hsa-Mir-223_3p","Hsa-Mir-224_5p","Hsa-Mir-296_5p","Hsa-Mir-320-P1_3p","Hsa-Mir-320-P2a_3p","Hsa-Mir-320-P2b_3p","Hsa-Mir-320-P3_3p","Hsa-Mir-320-P4_3p","Hsa-Mir-324_5p","Hsa-Mir-325_3p","Hsa-Mir-326_3p","Hsa-Mir-328_3p","Hsa-Mir-330-v1_3p","Hsa-Mir-330-v2_3p","Hsa-Mir-331_3p","Hsa-Mir-335_5p","Hsa-Mir-335_3p","Hsa-Mir-337-as_5p","Hsa-Mir-337-as_3p","Hsa-Mir-337-v1_5p","Hsa-Mir-337-v1_3p","Hsa-Mir-337-v2_5p","Hsa-Mir-337-v2_3p","Hsa-Mir-338-P2_3p","Hsa-Mir-338-P2-as_5p","Hsa-Mir-338-P2-as_3p","Hsa-Mir-339_5p","Hsa-Mir-339_3p","Hsa-Mir-340_5p","Hsa-Mir-342_3p","Hsa-Mir-345_5p","Hsa-Mir-346_5p","Hsa-Mir-361-v1_5p","Hsa-Mir-361-v2_3p","Hsa-Mir-362-P1_5p","Hsa-Mir-362-P2-v1_3p","Hsa-Mir-362-P2-v2_3p","Hsa-Mir-362-P3_3p","Hsa-Mir-362-P4_3p","Hsa-Mir-362-P5_5p","Hsa-Mir-370_3p","Hsa-Mir-374-P1_5p","Hsa-Mir-374-P1_3p","Hsa-Mir-374-P2_5p","Hsa-Mir-375_3p","Hsa-Mir-376-P1_3p","Hsa-Mir-376-P2_3p","Hsa-Mir-376-P3_3p","Hsa-Mir-376-P4_3p","Hsa-Mir-376-P5_3p","Hsa-Mir-378_3p","Hsa-Mir-383-v1_5p","Hsa-Mir-383-v2_5p","Hsa-Mir-423_5p","Hsa-Mir-423_3p","Hsa-Mir-425_5p","Hsa-Mir-430-P1_3p","Hsa-Mir-430-P2_3p","Hsa-Mir-430-P3_3p","Hsa-Mir-430-P4_3p","Hsa-Mir-430-P5_5p","Hsa-Mir-430-P5_3p","Hsa-Mir-430-P6_3p","Hsa-Mir-430-P7_3p","Hsa-Mir-430-P8a_3p","Hsa-Mir-430-P8b_3p","Hsa-Mir-430-P9a_5p","Hsa-Mir-430-P9a_3p","Hsa-Mir-430-P9b_5p","Hsa-Mir-430-P9b_3p","Hsa-Mir-430-P10_3p","Hsa-Mir-430-P11_3p","Hsa-Mir-430-P12_5p","Hsa-Mir-430-P13_5p","Hsa-Mir-430-P14_3p","Hsa-Mir-430-P15_5p","Hsa-Mir-430-P16_3p","Hsa-Mir-430-P17_5p","Hsa-Mir-430-P18_5p","Hsa-Mir-430-P18_3p","Hsa-Mir-430-P19_3p","Hsa-Mir-430-P21_3p","Hsa-Mir-430-P22_3p","Hsa-Mir-430-P23_3p","Hsa-Mir-430-P24_3p","Hsa-Mir-430-P25_3p","Hsa-Mir-430-P26_3p","Hsa-Mir-430-P27a_3p","Hsa-Mir-430-P27b_3p","Hsa-Mir-430-P29a_3p","Hsa-Mir-430-P29b_3p","Hsa-Mir-430-P31a_5p","Hsa-Mir-430-P31b_5p","Hsa-Mir-430-P33_5p","Hsa-Mir-430-P34_5p","Hsa-Mir-430-P35a_3p","Hsa-Mir-430-P35b_3p","Hsa-Mir-430-P35c_3p","Hsa-Mir-430-P38_3p","Hsa-Mir-430-P39_3p","Hsa-Mir-430-P41_3p","Hsa-Mir-430-P42_3p","Hsa-Mir-430-P43_5p","Hsa-Mir-430-P43_3p","Hsa-Mir-430-P44_5p","Hsa-Mir-430-P45_5p","Hsa-Mir-430-P46_5p","Hsa-Mir-430-P47_5p","Hsa-Mir-430-P48_5p","Hsa-Mir-430-P49_5p","Hsa-Mir-430-P50_5p","Hsa-Mir-431_5p","Hsa-Mir-431_3p","Hsa-Mir-432_5p","Hsa-Mir-433_3p","Hsa-Mir-448_5p","Hsa-Mir-448_3p","Hsa-Mir-450-P1_5p","Hsa-Mir-450-P2_5p","Hsa-Mir-450-P3_5p","Hsa-Mir-451_5p","Hsa-Mir-452-v1_5p","Hsa-Mir-452-v2_5p","Hsa-Mir-455_5p","Hsa-Mir-459_5p","Hsa-Mir-483_5p","Hsa-Mir-486_5p","Hsa-Mir-488_3p","Hsa-Mir-489-v1_3p","Hsa-Mir-489-v2_3p","Hsa-Mir-490_5p","Hsa-Mir-491_5p","Hsa-Mir-493_5p","Hsa-Mir-499_5p","Hsa-Mir-504-v1_5p","Hsa-Mir-504-v2_5p","Hsa-Mir-505-v1_5p","Hsa-Mir-505-v2_5p","Hsa-Mir-506-P1_5p","Hsa-Mir-506-P1_3p","Hsa-Mir-506-P2_3p","Hsa-Mir-506-P3_3p","Hsa-Mir-506-P4a1_3p","Hsa-Mir-506-P4a2_3p","Hsa-Mir-506-P4b_3p","Hsa-Mir-506-P5_5p","Hsa-Mir-506-P6a1_5p","Hsa-Mir-506-P6a2_5p","Hsa-Mir-506-P6b_5p","Hsa-Mir-506-P6c_5p","Hsa-Mir-506-P7a_3p","Hsa-Mir-506-P7b_3p","Hsa-Mir-506-P7c_3p","Hsa-Mir-506-P8_5p","Hsa-Mir-506-P9_5p","Hsa-Mir-506-P10_5p","Hsa-Mir-506-P11_3p","Hsa-Mir-506-P12_3p","Hsa-Mir-506-P13_5p","Hsa-Mir-506-P13_3p","Hsa-Mir-506-P29_3p","Hsa-Mir-511_5p","Hsa-Mir-511_3p","Hsa-Mir-541_5p","Hsa-Mir-541_3p","Hsa-Mir-542-v1_3p","Hsa-Mir-542-v2_3p","Hsa-Mir-544_3p","Hsa-Mir-550-P1_3p","Hsa-Mir-550-P2a_3p","Hsa-Mir-550-P2b_3p","Hsa-Mir-551-P1_3p","Hsa-Mir-551-P2_3p","Hsa-Mir-552_5p","Hsa-Mir-552_3p","Hsa-Mir-556_5p","Hsa-Mir-556_3p","Hsa-Mir-561_5p","Hsa-Mir-574_3p","Hsa-Mir-576_5p","Hsa-Mir-577_5p","Hsa-Mir-579_3p","Hsa-Mir-580_3p","Hsa-Mir-582-v1_5p","Hsa-Mir-582-v2_5p","Hsa-Mir-584_5p","Hsa-Mir-585_3p","Hsa-Mir-589_5p","Hsa-Mir-590_3p","Hsa-Mir-592_5p","Hsa-Mir-598_3p","Hsa-Mir-599_5p","Hsa-Mir-605-v1_3p","Hsa-Mir-605-v2_3p","Hsa-Mir-615_3p","Hsa-Mir-616_5p","Hsa-Mir-624_5p","Hsa-Mir-628_5p","Hsa-Mir-628_3p","Hsa-Mir-629_5p","Hsa-Mir-642_5p","Hsa-Mir-651_5p","Hsa-Mir-652_3p","Hsa-Mir-653_3p","Hsa-Mir-670_3p","Hsa-Mir-671_5p","Hsa-Mir-671_3p","Hsa-Mir-675_3p","Hsa-Mir-676_3p","Hsa-Mir-744_5p","Hsa-Mir-760_3p","Hsa-Mir-766_3p","Hsa-Mir-769_5p","Hsa-Mir-770_5p","Hsa-Mir-873-v1_5p","Hsa-Mir-873-v1_3p","Hsa-Mir-873-v2_5p","Hsa-Mir-874_3p","Hsa-Mir-875_5p","Hsa-Mir-876_3p","Hsa-Mir-877_5p","Hsa-Mir-885_5p","Hsa-Mir-887_5p","Hsa-Mir-887_3p","Hsa-Mir-935-v1_3p","Hsa-Mir-935-v2_3p","Hsa-Mir-944_3p","Hsa-Mir-1180_3p","Hsa-Mir-1247_5p","Hsa-Mir-1249_3p","Hsa-Mir-1251_5p","Hsa-Mir-1252_5p","Hsa-Mir-1266_5p","Hsa-Mir-1269_3p","Hsa-Mir-1270_5p","Hsa-Mir-1271_5p","Hsa-Mir-1271_3p","Hsa-Mir-1276_5p","Hsa-Mir-1277_5p","Hsa-Mir-1278_3p","Hsa-Mir-1286_3p","Hsa-Mir-1287_5p","Hsa-Mir-1294_5p","Hsa-Mir-1295_3p","Hsa-Mir-1296_5p","Hsa-Mir-1298_5p","Hsa-Mir-1301_3p","Hsa-Mir-1306_5p","Hsa-Mir-1307_5p","Hsa-Mir-1323_5p","Hsa-Mir-1343_3p","Hsa-Mir-1908_5p","Hsa-Mir-1910_3p","Hsa-Mir-1911_5p","Hsa-Mir-1912_3p","Hsa-Mir-1913_5p","Hsa-Mir-1913_3p","Hsa-Mir-1914_5p","Hsa-Mir-2114_5p","Hsa-Mir-2114_3p","Hsa-Mir-2115_3p","Hsa-Mir-2116_5p","Hsa-Mir-2116_3p","Hsa-Mir-2276_5p","Hsa-Mir-2276_3p","Hsa-Mir-2355_5p","Hsa-Mir-2355_3p","Hsa-Mir-2467_5p","Hsa-Mir-3059_5p","Hsa-Mir-3085_3p","Hsa-Mir-3126_5p","Hsa-Mir-3127_5p","Hsa-Mir-3136_5p","Hsa-Mir-3138_3p","Hsa-Mir-3140_3p","Hsa-Mir-3144-v1_5p","Hsa-Mir-3144-v2_5p","Hsa-Mir-3145_3p","Hsa-Mir-3146_3p","Hsa-Mir-3173_5p","Hsa-Mir-3174_5p","Hsa-Mir-3180-P1_3p","Hsa-Mir-3180-P2_3p","Hsa-Mir-3180-P3_3p","Hsa-Mir-3187_3p","Hsa-Mir-3194_5p","Hsa-Mir-3200_3p","Hsa-Mir-3611_3p","Hsa-Mir-3613_5p","Hsa-Mir-3617_5p","Hsa-Mir-3619_5p","Hsa-Mir-3622_5p","Hsa-Mir-3622_3p","Hsa-Mir-3663_3p","Hsa-Mir-3690_5p","Hsa-Mir-3909_3p","Hsa-Mir-3924_3p","Hsa-Mir-3934_5p","Hsa-Mir-3934_3p","Hsa-Mir-3942_5p","Hsa-Mir-3942_3p","Hsa-Mir-3944_3p","Hsa-Mir-4424_3p","Hsa-Mir-4524_3p","Hsa-Mir-4637_3p","Hsa-Mir-4661_5p","Hsa-Mir-4662_5p","Hsa-Mir-4664_5p","Hsa-Mir-4664_3p","Hsa-Mir-4677_3p","Hsa-Mir-4731_5p","Hsa-Mir-4731_3p","Hsa-Mir-4766_5p","Hsa-Mir-4766_3p","Hsa-Mir-5579_5p","Hsa-Mir-5581_5p","Hsa-Mir-5581_3p","Hsa-Mir-5586_5p","Hsa-Mir-5683_5p","Hsa-Mir-5687_5p","Hsa-Mir-5687_3p","Hsa-Mir-6715_3p","Hsa-Mir-7151_5p","Hsa-Mir-7151_3p","Hsa-Mir-7854_3p","Hsa-Novel-1-v1_3p","Hsa-Novel-2_5p","Hsa-Novel-2_3p","Hsa-Novel-3_5p"
"ERR409886",18482,47783,208285,208285,208285,129115,129115,27498,21535,7913,16405,45477,89,1,89,1932,1932,1932,381,46,389,349,466,162369,8029,162369,8029,162369,8029,33274,2402,3269,551,84391,22426,52166,181833,85271,85271,3166,363,352,2111,36647,37811,20,5760,662,164,473,254,104,28,884,163,3226,399,1216,1216,21918,4210,9845,3637,3329,3329,1006516,30890,1006516,163575,11134,1438,14574,29328,6468,1337,6548,2989,6548,2989,43894,11586,79701,187929,81149,14185,36070,36070,949,293,433,323,967,79,17,589,7,8,7,0,16,22290,22290,78218,1,2416,5989,994,2462,10,8,4,25,217,7399,1483,590,40121,31860,40121,31860,7270,35489,35489,78,78,5,41977,52733,41977,52733,41977,52733,24196,106512,25090,128705,72327,72327,2106,3709,546,4454,518,3843,1086,190,287,6120,302,753,1559,508,52,9,1559,508,720,1351,1351,8,9448,1723,1608,2194,254,11997,11997,6497,4501,8186,1835,76,367,2979,620467,1162,1282,7301,17722,967,4,5,11049,191,3009,10014,1904,11572,11572,268,20,85,59,1103,2084,1134,502,502,367,493,450,432,27,209,11651,412,7405,9289,20935,92,840,462,2164,87,424,19,18,1622,309,4200,62,379,515,2947,18,355,4,0,149,3582,695,392597,392597,43191,22462,22462,5225,141,285,25940,79,26,1276,1129,2275,18,119626,902,8147,410,177,74,465,574,580,16,12,19,6654,3161,3637,359,3161,3637,359,3161,3637,359,0,370,90,174,18936,9,56,1,456,79,121,0,0,0,3,3644,3644,2830,1575,354,2139,4,11573,888,17482,869,122,20,2912,284,284,74,79,597,0,73,327,750,960,920,4664,1975,2,10,10,35,24,63,21870,21979,248,96,1043,8654,6748,1105,247,2537,1202,196,1259,987,711,213,23,609,804,1278,863,373,217,37,217,911,4,7172,266,313,3003,8642,2484,2,1,7,6,0,3,1,4,0,0,3,0,3,0,4,3,0,2,0,8,23,2,2,1,3,0,2,2,1,0,0,0,0,0,0,127,127,20,1,2,2,3,0,0,30,0,0,6,0,0,0,0,3,8,8,16,27,1650,1122,3,7,98,98,181,9006,41,36,263,0,84,21802,607,25,3,26,70,36,132,672,582,5,0,0,1,0,5,13,13,13,0,1,1,0,2,2,2,2,0,6,1,7,0,0,1,0,8,6,9,12,42,36,6,57,57,57,6,63,2,2,2,0,90,1141,70,1294,0,6,280,125,610,3,333,83,188,2777,0,2,0,10,17,6,847,372,63,3,33,178,1,62,78,443,7,1,4015,21,274,20009,470,1630,309,1077,2891,2,38,192,1026,22,841,944,975,2,1489,126,914,77,19,0,3,7,799,0,9,76,1,36,17,1,1,2648,284,370,49,9072,1,32,21,0,40,18,1,4,1,9,6,9,1,5,0,1,38,1,163,74,8,0,13,1,2,0,0,0,0,0,4,1,16,16,16,0,6,719,14,143,2,1,8,16,0,4,74,0,1,0,1,0,0,10,0,0,0,44,0,1,33,0,1,0,2,0,0,4,23,427,0,0,1,0,3,4,0,1,2,1
"ERR409894",25295,74145,314056,314056,314056,196868,196868,44708,38319,12313,25733,73578,80,0,80,2544,2544,2544,60,12,52,47,23,296685,5669,296685,5669,296685,5669,61620,4875,1558,331,41100,14486,30986,81252,66568,66568,4681,473,316,3132,43675,45066,17,4206,806,114,446,364,131,17,1169,170,4443,296,1181,1181,16810,3742,11247,2053,3471,3471,1077711,36069,1077711,251199,4538,962,10434,38256,15234,1483,9233,3091,9233,3091,40611,13395,112919,181079,77627,20833,38564,38564,1215,336,1173,523,1588,102,15,992,11,20,2,0,18,35052,35052,64729,0,1835,7599,1128,4416,11,9,2,24,9,1411,50,26,49727,59678,49727,59678,12143,60887,60887,146,146,24,59511,69508,59511,69508,59511,69508,13765,73971,14378,181488,87393,87393,2443,7258,857,3754,448,6669,1345,93,269,10423,319,828,1134,372,39,2,1134,372,1886,1296,1296,8,16570,2736,1479,2334,206,28543,28543,8033,4699,8944,1127,44,190,1110,197946,893,1024,3502,25612,1136,15,16,6976,173,3722,16591,1010,12486,12486,349,73,118,109,2897,4897,1953,747,747,887,868,455,1055,34,233,7366,1364,27279,17203,36872,35,1618,1180,3475,225,637,42,75,2579,264,2381,142,595,1042,5190,79,896,8,0,303,10352,306,1210291,1210291,62245,81344,81344,7834,212,430,25963,115,26,1259,1342,2729,17,135192,941,8565,694,243,67,359,519,532,12,11,16,4628,1120,1313,122,1120,1313,122,1120,1313,122,0,669,143,13,12255,5,126,0,575,15,19,0,9,4,3,5260,5260,10450,8358,2101,7874,2,16851,496,23335,301,31,33,3306,433,433,113,116,1079,0,93,471,1467,1740,1499,3550,1611,2,3,3,47,16,69,60609,60760,352,154,1462,32764,6652,1518,385,2464,1797,137,985,829,292,114,22,979,940,2096,931,1010,278,35,278,1288,0,5042,699,901,2677,6997,4201,7,4,8,6,0,0,0,4,1,1,6,0,6,0,3,0,0,1,0,18,20,1,1,2,2,2,0,0,0,0,0,0,0,1,1,123,123,2,1,2,2,4,2,2,16,0,0,14,0,0,0,1,0,9,9,76,90,3853,2099,0,6,99,99,210,17440,7,5,161,1,11,20637,1413,30,7,91,175,59,227,607,568,10,2,0,1,0,10,13,13,13,0,4,4,1,7,16,16,16,0,0,0,5,0,0,1,0,7,2,11,66,112,51,8,29,29,29,2,76,5,0,1,1,25,813,60,3218,2,3,469,238,2573,1,597,92,615,4342,0,0,0,8,18,1,1402,444,54,7,57,253,6,136,151,705,1,7,8443,48,226,59904,413,3923,880,2508,4247,0,59,464,1511,24,1237,1708,1775,0,2620,103,1958,183,15,4,6,10,225,2,13,75,0,55,55,1,0,2771,95,435,80,13812,5,59,56,0,9,3,1,4,3,4,2,16,0,7,1,9,38,3,352,163,37,0,12,0,0,0,0,0,0,2,6,0,8,8,8,5,0,1544,11,384,2,0,18,10,5,0,94,0,0,0,2,0,2,11,2,0,2,69,0,0,104,2,0,1,0,0,0,7,6,497,0,1,0,9,20,4,2,2,0,1
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"ERR409890",31807,73537,332584,332584,332584,207846,207846,40131,39626,13528,27316,82526,123,0,123,4122,4122,4122,86,16,51,49,40,313212,9454,313212,9454,313212,9454,12711,916,1025,192,70976,18562,40955,140567,80308,80308,5146,376,297,3161,42388,43780,20,5432,659,146,559,299,102,30,1086,209,3821,219,964,964,18528,6017,8092,2176,3320,3320,880724,34280,880724,248387,7578,1316,11128,42063,12966,1498,11360,4032,11360,4032,52719,16568,105362,219067,104021,22854,47955,47955,1369,356,893,481,1860,58,9,275,9,21,3,0,29,24237,24237,70504,0,2615,7057,1625,3681,4,5,8,30,36,1357,119,58,55407,59213,55407,59213,13381,58842,58842,146,146,25,54009,65570,54009,65570,54009,65570,16738,97910,17466,190691,107450,107450,3684,7559,1321,5110,815,7089,1475,155,392,11625,494,1157,1379,412,50,5,1379,412,1050,2118,2118,23,18360,3196,1995,2769,288,24021,24021,8118,4202,8106,1381,64,208,1481,229590,1142,1285,2522,34626,1152,12,22,6384,139,3829,13252,1419,16663,16663,311,47,144,127,1585,2864,1684,572,572,803,1075,513,930,31,305,13012,1018,25557,15845,34339,25,1259,1120,3402,165,759,42,45,2849,414,3448,115,768,755,6720,46,1027,6,1,340,6618,405,765015,765015,71930,51222,51222,8095,245,529,27633,147,26,1121,1207,3130,15,139265,1164,9463,432,226,104,527,655,660,9,6,13,4062,915,1062,98,915,1062,98,915,1062,98,0,988,243,215,19871,5,140,0,356,11,18,4,4,0,3,6779,6779,7676,3932,1265,6585,1,16620,1060,27362,401,26,15,3065,413,413,141,148,887,2,81,404,1039,1287,1257,5320,2358,2,12,12,40,36,65,49868,50027,323,57,1168,22978,6487,1275,285,2813,1532,216,1049,862,589,153,28,791,859,1718,819,644,248,60,248,1243,7,5638,332,394,2588,6878,3842,3,1,3,5,15,3,16,26,0,0,17,0,17,0,1,0,0,4,0,14,29,4,4,3,0,0,1,1,1,0,0,0,0,0,0,130,130,1,0,4,4,0,0,0,56,0,0,12,0,0,0,0,0,12,12,44,60,2793,2111,2,7,172,172,384,14091,11,8,174,1,8,18780,1484,67,12,48,107,83,218,737,655,9,1,0,1,0,9,7,7,7,0,1,1,0,3,8,8,8,0,5,3,11,3,0,0,0,3,3,15,19,129,85,12,45,45,45,0,57,4,4,5,2,52,649,48,2817,1,6,545,226,1389,5,449,97,595,3643,0,1,2,4,28,2,1642,452,38,13,53,190,4,119,117,571,1,6,7249,34,353,44263,438,3630,629,2398,3490,0,83,253,1655,39,905,1266,1332,1,2277,97,1083,208,33,0,9,12,596,3,10,83,5,57,28,2,0,2199,57,518,43,7089,0,37,55,0,2,2,0,5,2,11,9,8,1,6,1,6,27,3,226,126,26,0,12,1,0,0,0,0,0,0,0,4,6,6,6,3,1,844,5,271,1,0,8,12,3,4,88,0,0,0,1,0,0,16,1,0,3,104,0,0,91,0,0,0,1,3,0,5,27,563,0,0,2,2,7,2,0,0,1,2
"ERR409892",20070,62698,249356,249356,249356,133797,133797,28694,25804,9305,15145,41838,24,1,24,1976,1976,1976,139,25,44,81,27,190994,10681,190994,10681,190994,10681,12178,810,3019,549,58992,24577,38033,180845,103672,103672,4126,418,354,3333,49614,51047,16,6714,590,82,439,189,69,16,649,88,2346,346,1312,1312,13654,5602,18031,3275,3630,3630,1170207,33901,1170207,172564,8606,1527,9062,38068,7155,2654,7076,4117,7076,4117,64033,18645,101044,139165,77035,19332,44324,44324,1313,267,337,332,1222,52,17,390,5,20,12,0,35,17825,17825,62383,3,1620,5127,1296,5477,16,20,1,24,19,587,114,80,27676,31360,27676,31360,10800,50157,50157,145,145,14,63271,79153,63271,79153,63271,79153,35600,136276,36538,174992,104744,104744,4124,4919,1408,6404,638,6635,1358,111,240,12518,568,1160,1639,585,50,2,1639,585,1945,1927,1927,37,12415,2209,2315,3653,437,23327,23327,9671,2442,5579,909,55,209,795,281332,325,368,7829,31389,1307,15,17,10277,177,6456,17032,2086,19234,19234,382,94,140,116,3133,5944,2168,1035,1035,743,912,712,716,22,388,5763,1080,21698,14205,29878,240,1675,1708,3401,234,708,51,75,3695,694,3043,101,1408,1673,4649,92,757,5,0,411,5220,462,459187,459187,46664,27527,27527,5887,210,226,23968,93,25,1033,1174,2269,14,171479,929,7783,790,129,97,693,560,572,5,3,5,7292,2284,2596,259,2284,2596,259,2284,2596,259,0,690,156,146,19024,5,130,0,481,51,63,2,4,2,0,4535,4535,3557,1694,463,2327,2,17120,1196,24404,694,74,16,2067,450,450,119,121,857,0,81,528,1064,1413,1299,9158,2876,1,6,4,31,11,47,31241,31347,268,115,1318,13467,11001,1466,413,4208,1499,99,1104,885,613,98,16,853,1171,1295,1386,876,297,39,297,1295,3,3634,351,409,2456,9939,2756,6,0,4,2,0,1,1,0,0,0,2,0,2,0,0,0,0,2,0,6,29,2,2,0,0,0,0,0,0,0,0,0,0,0,0,182,182,7,3,0,0,1,0,0,57,0,0,7,1,0,0,2,0,9,9,56,51,3519,1879,2,9,102,102,331,2908,31,16,330,0,53,6796,2129,62,7,71,212,53,185,1014,899,4,1,0,3,0,21,23,23,23,1,3,3,1,1,20,20,20,0,4,0,11,0,0,0,0,8,8,19,17,59,29,5,54,54,54,4,82,2,1,2,1,24,743,37,1173,0,5,656,336,651,3,391,126,486,2952,38,1,0,1,16,3,2528,791,30,2,39,198,2,106,104,613,1,8,8185,30,259,35890,563,3828,1037,2360,4521,3,64,330,1771,22,885,1848,1925,3,1561,108,2026,241,15,1,4,6,403,1,19,128,0,36,47,3,0,4381,46,389,76,5298,0,54,65,3,9,7,2,3,4,21,10,11,0,2,0,5,23,1,171,166,58,0,6,0,0,1,1,0,0,0,2,0,1,1,1,3,1,1213,10,131,3,0,9,1,0,14,68,0,1,0,5,0,1,15,1,0,0,59,0,0,85,0,1,0,0,0,0,4,12,183,0,0,0,4,1,2,0,0,0,0
"ERR409888",20686,53365,320237,320237,320237,154352,154352,56350,35260,10690,24807,53362,33,0,33,1368,1368,1368,66,6,44,21,16,160209,4621,160209,4621,160209,4621,603,38,773,135,55847,11674,53908,87696,61217,61217,4751,527,386,1869,56121,57923,18,4529,934,62,510,403,222,15,1176,93,6879,358,1284,1284,14586,3041,28252,2087,3465,3465,839104,33080,839104,422947,4270,894,8432,57885,20470,1302,3688,2158,3688,2158,26618,8055,77062,101046,55954,13633,26101,26101,873,354,1031,166,683,177,56,1336,4,6,2,0,10,41615,41615,33066,2,1010,8566,735,6452,12,14,3,17,13,811,41,14,31455,39013,31455,39013,9413,50436,50436,75,75,2,17624,22097,17624,22097,17624,22097,13641,38517,14052,86092,43830,43830,2068,4274,1014,2841,467,6222,1645,124,371,11746,205,779,785,231,40,2,785,231,802,805,805,16,6748,971,546,1363,174,14740,14740,6313,5682,10425,720,38,145,1183,78666,259,309,2082,25018,1047,11,15,2906,120,3632,9871,871,4654,4654,214,23,63,57,1385,2505,1068,515,515,362,405,212,404,11,147,7493,524,12997,7498,18143,25,720,538,1735,138,568,13,35,1200,260,1705,60,313,429,2645,25,361,3,0,136,4378,340,1838301,1838301,41764,152459,152459,5508,224,290,27699,46,24,983,1068,1030,9,100751,752,7911,1072,139,147,533,397,412,4,0,0,6048,433,523,58,433,523,58,433,523,58,0,469,116,506,10508,7,37,0,455,5,10,3,6,1,1,2391,2391,13625,23548,5949,8819,4,9217,516,13915,403,7,35,4107,937,937,303,309,855,0,66,287,1817,2179,1294,2752,1210,1,7,7,26,12,36,108683,109010,824,147,740,25939,6245,969,315,2225,1719,109,819,695,282,89,9,517,750,1190,1147,499,161,18,161,576,4,4638,586,734,2498,6309,2966,0,0,0,3,6,3,14,18,0,0,2,0,2,0,0,1,0,0,0,3,6,0,0,1,0,0,0,0,0,1,0,1,1,0,0,24,24,4,1,0,0,0,0,0,15,0,0,2,0,0,0,0,0,1,1,29,25,2337,1155,1,5,53,53,112,10767,9,5,126,0,6,7143,501,23,1,39,99,43,211,357,314,10,3,0,0,0,11,14,14,14,0,1,1,0,5,1,1,1,0,0,0,4,0,0,0,0,4,1,14,15,60,31,6,38,38,38,9,54,4,5,9,2,24,2179,87,11631,1,6,188,106,7185,2,441,75,319,3627,0,0,0,0,33,2,745,311,18,58,19,178,3,120,57,326,4,7,4876,40,190,39144,243,1567,331,985,3021,0,39,317,1216,26,1056,935,994,0,1199,28,1052,76,18,3,6,32,603,2,14,54,2,29,72,3,1,2724,119,296,51,3220,2,32,21,0,5,6,0,2,1,8,4,10,2,19,2,16,73,2,101,59,11,0,13,3,3,1,0,0,0,0,16,4,9,9,9,0,0,1284,3,81,10,0,5,7,14,14,85,0,3,0,0,0,0,5,2,1,1,30,0,0,98,0,0,0,0,0,0,9,6,213,0,0,1,7,10,2,0,0,3,0
"ERR409883",50124,117559,287216,287216,287216,150966,150966,100340,51970,9076,78984,50759,302,0,302,40367,40367,40367,181,26,225,201,59,1119735,27361,1119735,27361,1119735,27361,5018,316,3471,687,468097,64336,91004,268350,354990,354990,4998,284,895,4963,42614,44550,96,13652,1311,292,2499,2068,649,105,1269,469,10365,817,5402,5402,33324,10176,13571,5281,23971,23971,1911607,44636,1911607,407620,23050,1703,19193,33314,46720,8424,35669,11189,35669,11189,171798,57777,349596,563598,335059,32911,69221,69221,2883,491,1586,3812,4965,74,21,802,11,14,11,1,63,39084,39084,76677,0,3661,12219,3110,4234,19,17,16,268,56,3398,2523,1335,147985,189832,147985,189832,37333,208477,208477,571,571,35,498334,612748,498334,612748,498334,612748,62043,168531,63733,2090432,269080,269080,20258,64162,4986,13149,1937,7467,934,340,1205,20602,3495,2896,7158,2419,991,58,7158,2419,11113,3078,3078,65,47726,7408,14128,29901,1870,74909,74909,40377,19877,28272,3074,131,581,3413,1060230,1685,1921,10765,156907,14390,67,77,16279,1124,32150,77904,1688,51619,51619,1586,379,256,276,9602,13797,7483,4254,4254,704,1731,2030,9343,318,493,26271,6867,40611,18954,35032,817,4894,1261,6952,343,4678,122,152,1793,1535,7937,1151,780,5730,5347,169,1856,31,3,1592,29631,951,427957,427957,100468,66958,66958,20062,957,2401,52335,968,69,6524,3906,4684,25,650151,1497,23833,3063,529,471,1168,856,875,5,0,0,15747,2042,2337,221,2042,2337,221,2042,2337,221,1,3725,689,1400,50953,17,319,0,1971,87,137,9,57,10,11,20344,20344,22926,10814,5018,16382,3,61170,2346,87707,538,7,24,23708,4792,4792,1507,1515,4305,0,497,3006,4138,6203,10836,19210,6120,8,36,36,71,80,133,57358,57586,701,1067,3959,60034,63126,2948,1604,7940,5494,1473,2578,1889,1300,582,84,11665,2101,2616,1650,1248,2230,210,2230,11983,18,28375,1408,1585,6434,11302,11004,2,0,5,9,10,1,5,35,2,2,6,0,6,0,6,7,3,11,3,30,109,11,11,1,2,1,6,6,0,0,2,0,0,4,4,532,532,21,4,3,3,0,10,10,63,0,3,50,2,3,3,0,12,12,12,140,531,14287,7284,24,89,280,280,351,16557,24,27,941,0,72,48819,5988,36,1,661,2194,145,641,5311,4509,2,0,0,3,2,45,108,108,108,4,3,3,0,2,13,13,13,1,7,6,5,0,0,0,0,8,16,99,97,507,326,42,101,101,101,13,438,22,35,7,4,246,1742,48,3985,3,13,1169,1035,3764,14,1101,78,1212,8978,0,0,0,1,41,5,1220,750,467,14,131,1110,21,266,1341,1691,22,7,31197,422,1714,379207,2475,32833,10260,21435,34460,0,320,840,4514,122,3861,3534,3761,3,5786,255,2912,2242,63,18,42,39,174,12,69,147,0,512,114,2,14,16093,223,2379,219,96360,2,192,70,1,29,57,7,55,8,38,19,36,4,12,0,7,91,19,1664,386,142,3,46,0,0,0,0,0,0,0,4,2,158,158,158,6,10,5946,72,632,18,4,62,18,6,26,159,0,28,2,4,0,12,24,1,0,0,39,3,0,250,1,4,0,2,1,2,26,21,5035,3,3,0,3,5,8,0,0,1,3
"ERR409900",114606,271460,1258967,1258967,1258967,802974,802974,174495,142624,55680,97566,310818,449,2,449,20439,20439,20439,164,45,140,90,82,1160019,26481,1160019,26481,1160019,26481,1784,136,2827,530,223093,54743,131887,378490,235292,235292,16596,1905,1287,13171,177881,182861,44,23195,2793,575,1619,997,455,66,4785,876,19463,772,3276,3276,49412,16041,39170,8280,13975,13975,2066195,124098,2066195,999775,18861,3739,36351,153639,45029,5772,28289,10093,28289,10093,127958,41609,403791,585382,311134,71196,161776,161776,6805,1286,3451,1312,7548,408,84,2460,20,34,14,0,36,108578,108578,300449,0,7367,27213,4329,18039,51,37,36,118,58,3342,186,103,134743,168965,134743,168965,47477,252692,252692,535,535,69,150302,181910,150302,181910,150302,181910,46629,260473,49065,602166,333051,333051,12122,33485,4616,14620,2601,24631,5466,458,1361,60706,1678,5030,5166,1492,167,5,5166,1492,4476,9489,9489,148,58020,8347,5435,8629,1034,115714,115714,35419,19893,35601,4710,210,830,4827,497102,2252,2510,9263,134204,5168,23,29,19534,483,13217,53480,4833,43066,43066,1315,184,585,553,8569,14720,8291,2470,2470,3515,3396,1706,3964,70,1106,42961,4609,109192,64366,150646,123,5312,5111,14033,661,3690,215,296,10706,1698,10548,437,2398,3018,20627,214,3808,26,2,1150,33478,1115,4350668,4350668,228499,320102,320102,27194,689,2334,87752,665,101,3461,3804,10323,25,500036,3042,26345,2294,909,428,1924,1708,1737,9,1,3,20189,3434,4117,338,3434,4117,338,3434,4117,338,0,2537,615,1928,41926,32,318,1,1816,84,118,9,12,4,19,17955,17955,29759,32651,8088,21118,7,84282,3645,90702,1322,46,84,13284,1725,1725,672,681,3642,0,298,1480,5476,6658,5486,15309,8632,8,44,44,169,133,259,213137,213856,1507,336,4280,94473,28328,5058,1296,8817,5286,662,4059,3497,1270,386,91,4071,3045,5972,3084,2991,946,156,946,4217,18,21283,3045,3766,11448,27542,15958,11,1,4,10,5,1,8,17,0,0,10,0,10,0,9,4,1,2,0,21,55,2,2,3,4,0,0,0,1,0,4,1,1,2,2,192,192,9,1,6,6,0,2,2,50,0,3,12,2,1,1,2,3,9,9,367,316,12500,8927,23,37,395,395,763,62895,38,23,631,0,21,76857,4194,166,17,228,449,240,651,2017,1856,29,7,1,1,0,43,43,43,43,1,8,8,0,15,32,32,32,0,15,1,20,4,1,1,0,30,9,62,171,338,200,50,201,201,201,4,178,15,10,17,11,165,4232,262,17181,0,28,1533,714,11392,33,1782,303,2428,13519,1,0,0,2,81,15,4184,1386,164,50,146,694,10,887,646,2322,8,25,32413,158,1081,193939,1838,9853,2269,6412,14719,0,278,1590,7838,124,5040,6012,6372,5,9394,206,4752,975,97,8,24,53,1716,3,60,336,9,254,126,15,12,8235,482,2372,200,42483,1,315,261,1,6,39,2,4,5,19,19,52,4,29,2,14,282,12,951,664,73,3,49,5,0,1,1,0,2,2,26,10,52,52,52,4,7,5746,39,893,7,2,97,25,17,13,415,0,2,0,4,1,3,71,2,0,1,207,1,0,353,0,2,1,1,0,1,39,115,2549,0,0,1,22,16,16,0,1,2,1

Note: the “mature_counts.csv” needs to be transposed prior running the statistical analysis. This can be done either user the R script or using a script called “transpose_csv.py”.

Let’s copy the transpose_csv.py script to the working folder:

...

Code Block
cat mature_counts.txt
Code Block
microRNA	ERR409882ERR409886	ERR409879ERR409894	ERR409881ERR409882	ERR409884ERR409895	ERR409889	ERR409894ERR409899	ERR409887ERR409879	ERR409880ERR409885	ERR409890ERR409878ERR409881ERR409884	ERR409885ERR409887	ERR409886ERR409896	ERR409891	ERR409899ERR409880	ERR409893ERR409878	ERR409896ERR409898	ERR409895ERR409897	ERR409888ERR409893	ERR409892ERR409898ERR409890ERR409892	ERR409897ERR409888	ERR409883	ERR409900
hsaHsa-letLet-7a7-P1b_5p	36460818482	30565125295	71288029486	18217851987	56826944172	31405326036	17820123604	31812146550	33257968044	43295013902	54604915724	20828433998	35158629525	28992624585	41769529935	42139515501	53141719495	32022939737	24935431807	18691020070	24277420686	28720950124	1258946114606
hsaHsa-letLet-7a7-3pP1c_5p	34147783	18474145	16568342	111118227	257100021	13766085	4858426	136128501	105162724	28839989	20541506	8584952	10075858	4765606	11469995	10241283	10646267	16786159	8873537	8562698	10153365	262117559	439271460
hsaHsa-letLet-7a-2-3p	16	6	9	3	13	9	4	3	7	17	12	4	7	2	5	9	9	123	13
hsa-let-7b-5p	59417	52115	83857	27892	92339	44708	25678	46347	40131	61357	59795	27498	37230	43602	62467	45870	75630	56350	28694	25897	28396	100340	174494
hsa-let-7b-3p	1998	1476	2335	913	2239	1220	733	1260	955	2535	1771	894	1118	999	1404	1294	1670	2293	1343	764	793	1180	4682
hsa-let-7c-5p	68342	58425	162724	39989	100021	74145	41506	65606	73537	69994	128501	47783	75857	66085	86159	84951	118227	53364	62697	41282	46267	117557	271459
hsa-let-7c-3p	44	30	83	21	45	74	27	39	38	41	70	33	34	38	54	55	77	290	23	28	108	155
hsa-let-7d-5p	14861	12397	30556	7751	20819	12313	7833	11095	13528	15326	22746	7913	14405	14122	19831	17317	27318	10690	9305	8698	10109	9076	55680
hsa-let-7d-3p	3790	2659	4503	1886	3511	2827	1613	2269	2029	3311	3875	1999	2370	2978	3305	2460	5372	3015	2857	1747	1800	3443	8451
hsa-let-7e-5p	29486	23604	68044	13902	44172	25295	15724	24585	31807	29935	46550	18482	29525	26036	39737	33998	51987	20686	20070	15501	19495	50124	114605
hsa-let-7e-3p	207	201	385	159	207	196	121	200	158	229	393	195	198	150	248	154	285	199	185	141	168	512	686
hsa-let-7f-5p	211184	198778	456864	127386	276474	196866	123391	191072	207846	250192	376484	129113	231751	223462	288225	276677	425085	154352	133796	126870	155630	150954	802971
hsa-let-7f-1-3p	228	151	348	132	259	158	98	194	175	233	261	116	156	118	187	174	243	159	192	124	103	226	560
hsa-let-7f-2-3p	1462	1013	1818	743	1376	896	497	1118	962	1387	1999	783	1062	701	1100	1127	1215	642	1024	578	799	1847	3221
hsa-miR-15a-5p	7002	5486	9893	3651	12407	4681	3288	5599	5146	9213	7931	3166	4055	5151	5804	5614	12518	4751	4126	3567	3704	4998	16596
hsa-miR-15a-3p	2	1	0	3	5	3	0	2	0	6	0	0	1	0	1	0	1	09
hsa-miR-16-5p	49664	48381	111712	40311	83908	43677	39434	67420	42402	73300	73543	36662	45433	50446	54609	55368	106992	56124	49628	34812	35133	42637	177920
hsa-miR-16-1-3p	1	4	5	0	5	1	1	3	0	3	1	2	1	1	3	1	3	011	15
hsa-miR-17-5p	1091	945	1495	629	1971	806	445	1242	659	1301	1079	662	666	548	805	681	822	934	590	537	561	1311	2793
hsa-miR-17-3p	174	202	259	117	467	138	97	155	149	247	216	153	110	157	161	134	311	132	113	136	133	380	420
hsa-miR-18a-5p	326	194	174	97	403	131	65	168	102	327	137	104	104	76	115	98	101	222	69	85	74	649	455
hsa-miR-18a-3p	43	40	48	21	70	22	15	22	20	38	44	23	18	25	40	22	62	421	24	34	17	86
hsa-miR-19a-5p	6	7	6	11	30	7	3	2	4	12	3	4	7	2	4	2	6	010
hsa-miR-19a-3p	468	368	318	305	1082	296	150	505	219	542	473	399	298	232	247	237	307	358	346	253	259	817	772
hsa-miR-19b-1-5p	7	3	2	2	7	3	2	6	3	5	2	3	1	0	1	1	113	10
hsa-miR-19b-3p	1482	1097	1466	1147	3082	1181	539	1708	964	1773	1656	1216	1054	766	884	878	1046	1284	1312	788	912	5402	3276
hsa-miR-20a-5p	1615	1317	2220	902	3172	1169	461	1836	1086	2163	1632	884	1199	855	1156	1097	1036	1176	649	673	869	1269	4785
hsa-miR-20a-3p	9	6	4	2	14	5	3	3	4	6	6	7	3	1	2	5	7	96
hsa-miR-21-5p	17256	12662	17865	8313	24112	11145	5837	11293	11957	21365	20088	14302	13403	11423	12353	15500	17497	10842	8743	7668	9397	21234	33370
hsa-miR-21-3p	534	561	466	368	819	611	186	482	423	803	398	595	296	540	464	376	510	470	306	261	267	490	1435
hsa-miR-22-5p	573	372	551	242	421	360	161	359	385	483	562	219	293	160	357	346	231	406	363	229	229	998	1543
hsa-miR-22-3p	6526	3693	10360	2276	6428	3742	2958	3652	6017	4830	7321	4210	4172	4826	6177	5011	9282	3041	5602	3335	2297	10176	16041
hsa-miR-23a-5p	0	2	6	0	7	5	2	1	4	1	1	2	1	9	4	1	7	012	10
hsa-miR-23a-3p	1785	1388	3082	1146	2153	1286	847	2136	1556	1966	2835	2553	1797	1339	1637	1648	2208	1208	2269	894	1054	4353	5098
hsa-miR-24-1-5p	24	15	11	4	23	2	3	11	7	25	14	6	7	2	9	6	8	111	7	2	60	36
hsa-miR-24-3p	5206	4549	6172	2715	6773	3471	1544	4085	3320	5937	4608	3329	2943	2039	3436	2830	2510	3465	3630	3148	1886	23971	13975
hsa-miR-24-2-5p	1	0	9	3	2	3	2	3	1	3	7	6	3	3	11	2	1	114	7
hsa-miR-25-5p	6	2	5	1	6	5	2	1	1	4	1	2	0	2	1	1	4	07
hsa-miR-25-3p	10678	8254	14145	4943	16696	7599	4914	9122	7057	11815	10600	5989	5872	7239	8869	7667	14988	8566	5127	3997	5421	12219	27213
hsa-miR-26a-5p	942607	674879	1353549	460648	1173421	1104148	481683	1026301	905383	1514327	1284627	1028272	954763	588897	719907	838025	918553	861348	1194165	539098	578238	1940621	2160732
hsa-miR-26a-1-3p	33	13	38	11	23	15	5	13	19	12	19	21	11	7	26	15	114	4	11	92	67
hsa-miR-26b-5p	8873	8470	12404	6004	16179	7036	4039	10340	7269	13698	16293	6699	6824	5658	6723	8201	10241	8101	6964	5297	5465	12834	21565
hsa-miR-26b-3p	117	120	161	55	191	64	47	86	88	139	165	86	73	81	85	74	176	886	52	62	93	260
hsa-miR-27a-5p	10	5	6	3	3	47	3	6	4	13	5	12	4	5	12	2	7	347	5	3	15	22
hsa-miR-27a-3p	6316	6048	11563	4314	8880	4538	3946	7333	7578	8045	10429	11134	5353	7110	7001	7213	11208	4270	8606	4711	4313	23050	18861
hsa-miR-28-5p	1189	1003	2766	714	2114	963	798	1139	1316	1423	2938	1438	1285	1523	1462	1706	3236	894	1527	826	943	1703	3740
hsa-miR-28-3p	9931	8512	22673	5850	17423	10445	6004	12452	11139	12577	20995	14581	12398	12654	12736	12438	25137	8456	9078	4997	7356	19199	36389
hsa-miR-29a-5p	139	119	221	78	197	133	48	154	137	129	278	95	129	56	115	114	136	6165	76	76	402	302
hsa-miR-29a-3p	47851	40318	86030	31781	67338	40611	24682	43362	52719	43606	87923	43894	39369	32490	41001	40846	60529	26618	64033	30538	27862	171798	127958
hsa-miR-30a-5p	53741	58354	125902	46942	92089	61011	33253	92094	76030	69153	138612	76104	54456	41981	58085	62219	82200	31718	61622	27745	49837	268845	163743
hsa-miR-30a-3p	5559	4462	11786	3361	6961	6289	3173	7023	5990	6561	12540	4597	5335	4929	6342	5095	9863	3229	6463	2249	3918	16800	17563
hsa-miR-31-5p	203	200	445	273	239	161	134	182	235	193	407	107	191	189	205	135	240	9180	210	140	572	859
hsa-miR-31-3p	16	9	19	1	5	5	3	5	6	3	7	1	6	0	10	4	6	153	15
hsa-miR-32-5p	824	539	651	344	1123	336	205	575	356	895	663	293	333	324	431	450	604	354	267	305	247	491	1286
hsa-miR-32-3p	45	34	52	21	61	21	17	29	28	51	45	19	34	27	28	46	37	321	12	13	22	146
hsa-miR-33a-5p	652	573	664	327	1196	541	185	494	498	841	478	339	222	276	526	325	525	181	341	285	309	3858	1399
hsa-miR-33a-3p	114	83	116	44	123	62	31	67	70	109	109	31	39	38	83	57	72	753	34	49	115	233
hsa-miR-92a-1-5p	13	2	13	4	4	14	1	3	3	2	5	2	5	3	4	4	711	2	2	3	31
hsa-miR-92a-3p	42246	38723	50223	20238	68324	35052	19661	34443	24237	42942	41887	22290	22354	41671	37906	29984	79714	41615	17825	19775	19460	39084	108578
hsa-miR-93-5p	6901	5416	7821	3750	9831	4443	2768	5608	3821	7290	5275	3226	3474	3914	5811	4263	8262	6879	2346	2739	2781	10365	19463
hsa-miR-93-3p	28	10	38	8	42	9	6	15	18	19	20	22	15	6	25	10	19	122	12	9	47	47
hsa-miR-95-5p	84	53	122	46	126	58	26	68	88	83	113	62	76	39	65	82	77	386	32	48	546	217
hsa-miR-95-3p	1110	969	2741	778	1742	1128	644	1520	1625	1217	2296	994	1364	889	1325	1264	1222	735	1296	818	824	3110	4329
hsa-miR-96-5p	26	9	41	12	23	9	47	35	36	17	18	217	27	37	16	24	97	119	9	9	56	58
hsa-miR-98-5p	50824	30986	80693	21549	45532	38319	21471	33600	39626	48053	58479	21535	42277	31044	46855	45473	54780	35260	25804	22597	25577	51970	142624
hsa-miR-98-3p	719	484	911	349	492	393	221	427	478	485	1016	320	645	568	656	668	1103	258	449	299	332	292	1828
hsa-miR-99a-5p	15965	13513	33096	12728	25485	14486	9571	20179	18562	13461	23895	22426	15968	14763	15562	12327	16006	11674	24577	8407	8378	64336	54743
hsa-miR-99a-3p	302	365	621	291	422	335	185	376	358	436	591	258	303	210	318	287	439	240	294	178	251	1491	1198
hsa-miR-100-5p	79481	37070	63987	30825	107620	30986	18025	44661	40955	44899	60082	52166	39781	40535	41072	28735	28319	53908	38033	20461	14628	91004	131887
hsa-miR-100-3p	154	89	184	37	166	144	36	62	62	180	168	40	89	68	93	111	101	101	86	29	48	65	319
hsa-miR-101-5p	449	636	966	420	894	557	310	586	529	627	901	538	456	481	574	496	829	243	728	374	412	753	1422
hsa-miR-101-3p	19431	18440	29284	14575	31144	18471	7449	17823	18165	24323	34198	9800	13607	10769	15929	17565	21584	9448	9053	8167	13225	57684	46489
hsa-miR-29b-1-5p	126	99	298	100	157	143	45	118	143	112	198	62	135	94	151	129	119	83	119	80	62	417	520
hsa-miR-29b-3p	9231	10383	16612	7804	18075	9233	3548	12501	11360	9461	18227	6548	9338	4033	8918	6990	7204	3688	7076	5152	6062	35669	28289
hsa-miR-29b-2-5p	43	33	86	31	59	46	23	61	47	34	48	29	46	31	53	27	223	41	26	21	180	164
hsa-miR-103a-2-5p	47	32	74	21	36	45	22	46	53	36	64	26	25	20	25	30	218	56	21	18	244	149
hsa-miR-103a-3p	57341	56545	120575	45558	74052	60887	33067	65870	58842	65720	82673	35489	52492	42411	73148	55872	72334	50436	50157	29897	36824	208477	252692
hsa-miR-103a-1-5p	6	2	4	0	1	4	2	3	4	4	0	0	1	0	3	4	222	4
hsa-miR-105-5p	94	67	195	23	93	96	64	50	103	74	177	43	95	93	129	124	188	3109	59	41	370	360
hsa-miR-105-3p	7	5	16	4	14	6	7	5	11	13	12	5	7	2	8	5	11	650	44
hsa-miR-106a-5p	99	71	304	88	191	114	43	249	146	128	125	164	83	83	130	104	159	682	63	128	292	575
hsa-miR-106a-3p	0	0	0	0	0	0	0	0	0	0	0	0	1	0	1	0	0	00
hsa-miR-107	12864	12924	25287	10703	16544	12143	8198	13385	13381	13838	20856	7270	10120	10615	15478	12161	22185	9413	10800	6737	8071	37333	47477
hsa-miR-16-2-3p	13	13	23	3	28	11	11	22	15	22	19	11	8	21	15	14	68	110	10	32
hsa-miR-192-5p	10850	10721	16893	7812	16012	8565	5717	11078	9463	14552	16540	8147	7275	8285	9508	10099	11859	7911	7783	5007	6835	23833	26345
hsa-miR-192-3p	3	1	5	1	1	1	1	3	2	7	5	4	1	2	1	1	1	24
hsa-miR-196a-5p	0	0	19	3	11	11	0	9	6	0	5	12	1	8	0	0	30	011	0	1
hsa-miR-196a-1-3p	0	0	0	0	1	0	0	0	0	0	0	0	0	0	0	0	00
hsa-miR-197-5p	2	7	6	3	2	5	4	1	2	1	5	4	2	4	3	4	2	112	14
hsa-miR-197-3p	4641	5186	9789	4043	6037	4628	3665	6083	4062	5562	7040	6654	4797	5322	6538	4461	8605	6048	7292	3844	2793	15747	20189
hsa-miR-199a-5p	140	82	1008	145	269	206	165	646	153	249	885	715	383	207	241	300	643	9596	93	173	723	520
hsa-miR-199a-3p	939	837	5648	918	1410	1120	1047	3302	915	1580	6687	3161	2028	1693	1651	2319	4909	433	2284	496	1079	2042	3434
hsa-miR-208a-3p	0	0	0	0	0	0	0	1	0	1	0	1	0	0	0	0	1	01
hsa-miR-129-5p	6973	6508	13303	3986	6530	7258	3774	5453	7559	7194	7962	3709	6279	4931	10167	6221	6809	4274	4919	3070	4283	64162	33485
hsa-miR-129-1-3p	1734	1327	2143	840	1159	857	773	1283	1321	1454	1387	546	1278	486	1733	870	749	1014	1408	642	784	4986	4616
hsa-miR-148a-5p	89	66	372	67	142	114	63	176	85	120	403	132	115	70	100	135	181	576	41	72	266	286
hsa-miR-148a-3p	6130	5176	25339	4577	8570	6976	5268	12690	6384	7454	37987	11049	9217	4590	5783	8804	11497	2906	10277	2773	5167	16279	19534
hsa-miR-30c-5p	8513	6090	11549	4502	9475	6710	3768	8687	7097	7538	13893	6697	6948	4477	6579	5918	6878	5295	9006	3720	4738	18333	20643
hsa-miR-30c-2-3p	361	296	668	213	521	294	199	357	322	374	798	339	353	324	535	380	823	241	238	149	293	2099	11167-P1d_5p	208285	314056	364624	531423	568283	289927	305660	546059	712891	182180	178203	421398	351589	318124	432959	186915	242780	417695	332584	249356	320237	287216	1258967
Hsa-Let-7-P2a1_5p	208285	314056	364624	531423	568283	289927	305660	546059	712891	182180	178203	421398	351589	318124	432959	186915	242780	417695	332584	249356	320237	287216	1258967
Hsa-Let-7-P2a2_5p	208285	314056	364624	531423	568283	289927	305660	546059	712891	182180	178203	421398	351589	318124	432959	186915	242780	417695	332584	249356	320237	287216	1258967
Hsa-Let-7-P2a3_5p	129115	196868	211190	425095	276482	223463	198784	376491	456871	127391	123392	276681	231752	191076	250199	126873	155636	288225	207846	133797	154352	150966	802974
Hsa-Let-7-P2b1_5p	129115	196868	211190	425095	276482	223463	198784	376491	456871	127391	123392	276681	231752	191076	250199	126873	155636	288225	207846	133797	154352	150966	802974
Hsa-Let-7-P2b2_5p	27498	44708	59417	75630	92339	43602	52115	59795	83857	27892	25678	45870	37230	46347	61357	25897	28396	62467	40131	28694	56350	100340	174495
Hsa-Let-7-P2b3_5p	21535	38319	50824	54780	45533	31044	30986	58479	80694	21549	21471	45473	42277	33601	48053	22597	25577	46855	39626	25804	35260	51970	142624
Hsa-Let-7-P2c1_5p	7913	12313	14861	27318	20819	14122	12397	22746	30556	7751	7833	17317	14405	11095	15326	8698	10109	19831	13528	9305	10690	9076	55680
Hsa-Let-7-P2c2_5p	16405	25733	35925	46007	40731	26046	31814	52245	48422	22131	16863	28264	28404	30137	36839	16318	21184	38978	27316	15145	24807	78984	97566
Hsa-Let-7-P2c3_5p	45477	73578	72845	123354	109175	74340	71170	128485	173832	46596	39681	98399	83319	68711	96580	47999	55668	104358	82526	41838	53362	50759	310818
Hsa-Mir-1-P1_3p	89	80	74	94	90	52	118	226	236	66	47	190	181	97	118	57	79	187	123	24	33	302	449
Hsa-Mir-1-P2_3p	1	0	0	0	2	0	0	0	4	2	0	0	1	0	0	0	0	22
Hsa-Mir-1-P3_3p	89	80	74	94	90	52	118	226	236	66	47	190	181	97	118	57	79	187	123	24	33	302	449
Hsa-Mir-7-P1_5p	1932	2544	2622	2601	3681	1065	2172	3395	7532	1447	851	3483	3044	2989	3100	1450	1927	4548	4122	1976	1368	40367	20439
Hsa-Mir-7-P2_5p	1932	2544	2622	2601	3681	1065	2172	3395	7532	1447	851	3483	3044	2989	3100	1450	1927	4548	4122	1976	1368	40367	20439
Hsa-Mir-7-P4_5p	1932	2544	2622	2601	3681	1065	2172	3395	7532	1447	851	3483	3044	2989	3100	1450	1927	4548	4122	1976	1368	40367	20439
Hsa-Mir-8-P1a_3p	381	60	131	86	128	64	50	98	111	35	49	64	51	47	121	46	462	86	139	66	181	164
Hsa-Mir-8-P1b_3p	46	12	9	29	27	21	11	26	38	7	5	26	21	25	18	16	115	16	25	6	26	45
Hsa-Mir-8-P2a_3p	389	52	77	46	182	48	77	67	68	36	39	23	39	36	117	43	347	51	44	44	225	140
Hsa-Mir-8-P2b_3p	349	47	40	64	73	33	45	93	83	34	25	40	48	50	56	20	435	49	81	21	201	90
Hsa-Mir-8-P3a_3p	466	23	61	25	63	23	32	38	29	16	19	25	19	44	76	20	126	40	27	16	59	82
Hsa-Mir-9-P1_5p	162369	296685	325973	351690	348083	202181	244049	538146	505079	196178	145191	338071	320620	290747	375141	143367	221771	352483	313212	190994	160209	1119735	1160019
Hsa-Mir-9-P1_3p	8029	5669	8439	8239	12697	5564	6070	13606	11707	4898	4103	7300	7883	8820	7685	4557	5025	7405	9454	10681	4621	27361	26481
Hsa-Mir-9-P2_5p	162369	296685	325973	351690	348083	202181	244049	538146	505079	196178	145191	338071	320620	290747	375141	143367	221771	352483	313212	190994	160209	1119735	1160019
Hsa-Mir-9-P2_3p	8029	5669	8439	8239	12697	5564	6070	13606	11707	4898	4103	7300	7883	8820	7685	4557	5025	7405	9454	10681	4621	27361	26481
Hsa-Mir-9-P3_5p	162369	296685	325973	351690	348083	202181	244049	538146	505079	196178	145191	338071	320620	290747	375141	143367	221771	352483	313212	190994	160209	1119735	1160019
Hsa-Mir-9-P3_3p	8029	5669	8439	8239	12697	5564	6070	13606	11707	4898	4103	7300	7883	8820	7685	4557	5025	7405	9454	10681	4621	27361	26481
Hsa-Mir-10-P1b-v1_5p	33274	61620	590	20151	10343	7551	745	6828	33515	6965	653	1120	1043	46880	1104	315	30186	937	12711	12178	603	5018	1784
Hsa-Mir-10-P1b-v2_5p	2402	4875	39	1621	815	621	53	446	2722	504	47	90	75	3480	84	26	2067	70	916	810	38	316	136
Hsa-Mir-10-P1c-v1_5p	3269	1558	257	1767	896	405	556	6232	33628	726	1175	1313	599	2808	1498	287	686	1232	1025	3019	773	3471	2827
Hsa-Mir-10-P1c-v2_5p	551	331	40	374	173	69	81	906	6418	106	194	246	98	551	288	45	125	230	192	549	135	687	530
Hsa-Mir-10-P2b_5p	84391	41100	76456	45448	114204	53057	39073	64175	83922	43204	32872	38187	55062	69417	39121	25110	22826	65593	70976	58992	55847	468097	223093
Hsa-Mir-10-P2c_5p	22426	14486	15965	16006	25485	14763	13513	23895	33096	12728	9571	12327	15968	20179	13461	8407	8378	15562	18562	24577	11674	64336	54743
Hsa-Mir-10-P2d_5p	52166	30986	79481	28319	107620	40535	37070	60082	63987	30825	18025	28735	39781	44661	44899	20461	14628	41072	40955	38033	53908	91004	131887
Hsa-Mir-10-P3b_5p	181833	81252	138889	126317	224474	111938	83880	192590	206640	68002	66334	106078	137101	131928	88529	64913	62802	124192	140567	180845	87696	268350	378490
Hsa-Mir-10-P3c_5p	85271	66568	79205	77580	124928	63693	61766	105925	140562	44846	40183	57630	69745	78369	66116	41947	36981	73506	80308	103672	61217	354990	235292
Hsa-Mir-10-P3d_5p	85271	66568	79205	77580	124928	63693	61766	105925	140562	44846	40183	57630	69745	78369	66116	41947	36981	73506	80308	103672	61217	354990	235292
Hsa-Mir-15-P1a_5p	3166	4681	7002	12518	12407	5151	5486	7931	9893	3651	3288	5614	4055	5599	9213	3567	3704	5804	5146	4126	4751	4998	16596
Hsa-Mir-15-P1b_5p	363	473	516	719	857	312	563	822	1007	471	295	474	465	715	823	300	306	478	376	418	527	284	1905
Hsa-Mir-15-P1c_5p	352	316	560	205	511	120	250	567	557	282	115	277	305	289	572	111	197	282	297	354	386	895	1287
Hsa-Mir-15-P1d_5p	2111	3132	2437	6701	3326	3637	2450	3479	7965	2261	2204	3698	2989	3168	2864	1994	2213	4398	3161	3333	1869	4963	13171
Hsa-Mir-15-P2a_5p	36647	43675	49659	106970	83889	50438	48372	73527	111687	40310	39428	55347	45421	67394	73290	34801	35131	54599	42388	49614	56121	42614	177881
Hsa-Mir-15-P2b_5p	37811	45066	51704	108410	86937	51359	49837	76507	114249	41613	40251	57207	47166	69275	76485	35712	36475	55968	43780	51047	57923	44550	182861
Hsa-Mir-15-P2c_5p	20	17	8	17	22	5	10	22	34	13	3	9	17	17	17	10	911	20	16	18	96	44
Hsa-Mir-15-P2d_5p	5760	4206	4855	8138	6641	3753	4989	8525	12105	6397	3595	6472	6607	8438	7082	3257	4859	5490	5432	6714	4529	13652	23195
Hsa-Mir-17-P1a_5p	662	806	1091	822	1971	548	945	1079	1495	629	445	681	666	1242	1301	537	561	805	659	590	934	1311	2793
Hsa-Mir-17-P1c_5p	164	114	99	159	191	83	71	125	304	88	43	104	83	249	128	63	128	131	146	82	62	292	575
Hsa-Mir-17-P1d_5p	473	446	857	1033	1318	436	573	866	850	412	400	554	355	731	853	413	358	549	559	439	510	2499	1619
Hsa-Mir-17-P1d_3p	254	364	419	814	579	381	324	428	490	226	277	302	229	350	391	218	262	349	299	189	403	2068	997
Hsa-Mir-17-P2a_5p	104	131	326	101	403	76	194	137	174	97	65	98	104	168	327	85	7115	102	69	222	649	455
Hsa-Mir-17-P2c_5p	28	17	29	18	48	18	13	20	34	13	11	20	18	41	27	7	112	30	16	15	105	66
Hsa-Mir-17-P4a_5p	884	1169	1615	1036	3172	855	1317	1632	2220	902	461	1097	1199	1836	2163	673	869	1156	1086	649	1176	1269	4785
Hsa-Mir-17-P4c_5p	163	170	144	215	257	111	84	168	469	124	62	173	136	309	189	67	158	214	209	88	93	469	876
Hsa-Mir-17-P4d_5p	3226	4443	6901	8262	9831	3914	5416	5275	7821	3750	2768	4263	3474	5608	7290	2739	2781	5811	3821	2346	6879	10365	19463
Hsa-Mir-19-P1a_3p	399	296	468	307	1082	232	368	473	318	305	150	237	298	505	542	253	259	247	219	346	358	817	772
Hsa-Mir-19-P2a_3p	1216	1181	1482	1046	3082	766	1097	1656	1466	1147	539	878	1054	1708	1773	788	912	884	964	1312	1284	5402	3276
Hsa-Mir-19-P2c_3p	1216	1181	1482	1046	3082	766	1097	1656	1466	1147	539	878	1054	1708	1773	788	912	884	964	1312	1284	5402	3276
Hsa-Mir-21_5p	21918	16810	24378	23678	33803	20854	17441	28742	25992	11326	7940	22008	20342	16238	29409	10725	13724	17677	18528	13654	14586	33324	49412
Hsa-Mir-22-P1b_3p	4210	3742	6526	9282	6428	4826	3693	7321	10360	2276	2958	5011	4172	3652	4830	3335	2297	6177	6017	5602	3041	10176	16041
Hsa-Mir-23-P2_3p	9845	11247	17222	11499	14994	6013	11852	14345	16971	10756	9108	12375	14864	12411	28226	9190	7116	12957	8092	18031	28252	13571	39170
Hsa-Mir-23-P3_3p	3637	2053	2551	3093	2989	1923	1912	4189	4722	1657	1310	2482	2937	3303	3081	1396	1721	2606	2176	3275	2087	5281	8280
Hsa-Mir-24-P2_3p	3329	3471	5206	2510	6773	2039	4549	4608	6172	2715	1544	2830	2943	4085	5937	3148	1886	3436	3320	3630	3465	23971	13975
Hsa-Mir-24-P3_3p	3329	3471	5206	2510	6773	2039	4549	4608	6172	2715	1544	2830	2943	4085	5937	3148	1886	3436	3320	3630	3465	23971	13975
Hsa-Mir-26-P1_5p	1006516	1077711	912248	890275	1126662	568618	648819	1234738	1306207	444239	468744	808800	928138	992232	1475541	523073	559600	692502	880724	1170207	839104	1911607	2066195
Hsa-Mir-26-P2_5p	30890	36069	41809	42091	67728	28058	37424	71349	64732	24388	18438	40245	36161	47704	56249	22951	26013	36812	34280	33901	33080	44636	124098
Hsa-Mir-26-P4_5p	1006516	1077711	912248	890275	1126662	568618	648819	1234738	1306207	444239	468744	808800	928138	992232	1475541	523073	559600	692502	880724	1170207	839104	1911607	2066195
Hsa-Mir-27-P2_3p	163575	251199	483786	330699	646255	237548	386589	340718	456521	233163	136266	283426	222559	251724	638569	177823	176044	336033	248387	172564	422947	407620	999775
Hsa-Mir-27-P3_3p	11134	4538	6316	11208	8880	7110	6048	10429	11564	4314	3946	7213	5353	7333	8045	4711	4313	7001	7578	8606	4270	23050	18861
Hsa-Mir-28-P1_5p	1438	962	1189	3235	2113	1522	1002	2937	2762	713	798	1705	1285	1138	1421	826	943	1459	1316	1527	894	1703	3739
Hsa-Mir-28-P1_3p	14574	10434	9908	25120	17403	12641	8493	20984	22652	5836	5997	12424	12393	12443	12565	4986	7353	12725	11128	9062	8432	19193	36351
Hsa-Mir-28-P2_5p	29328	38256	69875	55300	108618	47514	51180	55486	74781	29302	26416	44621	34429	37379	72979	34109	26047	51962	42063	38068	57885	33314	153639
Hsa-Mir-28-P2_3p	6468	15234	25710	14939	35513	11334	17850	19264	18176	9379	6398	13050	9831	11713	24988	6739	9175	15407	12966	7155	20470	46720	45029
Hsa-Mir-28-P3_5p	1337	1483	1943	1113	1909	763	1537	2643	2515	1419	1089	1245	1553	1502	1909	1048	848	1801	1498	2654	1302	8424	5772
Hsa-Mir-29-P1b-v1_3p	6548	9233	9231	7204	18075	4033	10383	18227	16612	7804	3548	6990	9338	12501	9461	5152	6062	8918	11360	7076	3688	35669	28289
Hsa-Mir-29-P1b-v2_3p	2989	3091	3800	3802	5576	2351	3578	7360	6025	2854	1926	2931	3390	4447	3581	2432	2387	2846	4032	4117	2158	11189	10093
Hsa-Mir-29-P1d-v1_3p	6548	9233	9231	7204	18075	4033	10383	18227	16612	7804	3548	6990	9338	12501	9461	5152	6062	8918	11360	7076	3688	35669	28289
Hsa-Mir-29-P1d-v2_3p	2989	3091	3800	3802	5576	2351	3578	7360	6025	2854	1926	2931	3390	4447	3581	2432	2387	2846	4032	4117	2158	11189	10093
Hsa-Mir-29-P2b_3p	43894	40611	47851	60529	67338	32490	40318	87923	86030	31781	24682	40846	39369	43362	43606	30538	27862	41001	52719	64033	26618	171798	127958
Hsa-Mir-29-P2d_3p	11586	13395	16570	19337	23043	9869	13317	28764	27061	10062	7965	14105	14032	14217	14330	9693	9690	13547	16568	18645	8055	57777	41609
Hsa-Mir-30-P1a_5p	79701	112919	97895	141169	120517	88055	76955	157223	225215	57601	63546	110045	110091	100389	115458	56006	65757	124821	105362	101044	77062	349596	403791
Hsa-Mir-30-P1b_5p	187929	181079	154770	275225	282422	133689	153308	400714	388430	128120	98963	196102	201672	262121	206834	76514	156644	202675	219067	139165	101046	563598	585382
Hsa-Mir-30-P1d_5p	81149	77627	94091	119188	152861	69828	75424	156264	183906	64357	51187	96291	103617	103390	104392	53593	67159	95959	104021	77035	55954	335059	311134
Hsa-Mir-30-P2a_5p	14185	20833	19681	21785	22050	12162	16435	37164	40028	12170	10449	20531	20408	22148	21349	9002	13257	18881	22854	19332	13633	32911	71196
Hsa-Mir-30-P2b_5p	36070	38564	46605	79221	61147	44997	34395	89158	95562	23331	24714	50059	51940	48346	45494	23380	32108	54920	47955	44324	26101	69221	161776
Hsa-Mir-30-P2d_5p	36070	38564	46605	79221	61147	44997	34395	89158	95562	23331	24714	50059	51940	48346	45494	23380	32108	54920	47955	44324	26101	69221	161776
Hsa-Mir-31_5p	949	1215	1564	2112	1607	1396	1339	2686	3318	1531	1199	1243	1547	1283	1492	1219	1063	1899	1369	1313	873	2883	6805
Hsa-Mir-32_5p	293	336	824	604	1123	324	539	663	651	344	205	450	333	575	895	305	247	431	356	267	354	491	1286
Hsa-Mir-33-P1_5p	433	1173	2449	1384	4399	927	1858	985	1114	737	446	775	350	793	2830	949	653	1175	893	337	1031	1586	3451
Hsa-Mir-33-P3_5p	323	523	616	493	1112	253	521	462	632	298	179	310	209	458	787	263	294	494	481	332	166	3812	1312
Hsa-Mir-34-P1_5p	967	1588	1556	1645	2428	1377	1840	2643	4042	1174	910	1797	1995	1732	1737	1376	924	2212	1860	1222	683	4965	7548
Hsa-Mir-34-P2a_5p	79	102	253	82	238	73	206	145	102	90	49	106	73	77	373	61	6136	58	52	177	74	408
Hsa-Mir-34-P2a_3p	17	15	56	16	34	21	44	14	34	16	10	18	12	15	54	13	739	9	17	56	21	84
Hsa-Mir-34-P2b_5p	589	992	1597	611	1228	475	1403	678	751	520	417	807	405	310	2047	509	305	1064	275	390	1336	802	2460
Hsa-Mir-34-P3a-v1_5p	7	11	6	5	16	12	8	17	13	14	6	11	11	13	5	7	811	20
Hsa-Mir-34-P3a-v2_5p	8	20	14	23	19	24	12	31	28	26	15	18	25	16	6	10	221	20	6	14	34
Hsa-Mir-34-P3b_5p	7	2	2	10	16	7	25	5	7	7	11	7	10	7	3	1	912	2	11	14
Hsa-Mir-34-P3b_3p	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0	00