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#!/bin/bash -l #PBS -N nfrnaseq_QC #PBS -l select=1:ncpus=2:mem=4gb #PBS -l walltime=24:00:00 #work on current directory (folder) cd $PBS_O_WORKDIR #load java and set up memory settings to run nextflow module load java NXF_OPTS='-Xms1g -Xmx4g' nextflow run nf-core/rnaseq \ -profile singularity \ -r 3.12.0 \ --input samplesheet.csv \ --outdir results \ --genome GRCh38GRCm38-local \ --skip_trimming \ --skip_alignment \ --skip_pseudo_alignment |
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#!/bin/bash -l #PBS -N nfRNAseq #PBS -l select=1:ncpus=2:mem=4gb #PBS -l walltime=48:00:00 cd $PBS_O_WORKDIR module load java NXF_OPTS='-Xms1g -Xmx4g' nextflow run nf-core/rnaseq --input samplesheet.csv \ --outdir results \ -r 3.12.0 \ --genome GRCh38GRCm38-local \ -profile singularity \ --aligner star_salmon \ --extra_trimgalore_args "--quality 30 --clip_r1 10 --clip_r2 10 --three_prime_clip_r1 1 --three_prime_clip_r2 1 " |
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