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  1. A HPC account

  2. Nextflow installed on the HPC

  3. PuTTy installed on your local computer

  4. Access your HPC home directory from your PC

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If you haven’t installed Nextflow, follow the instructions in this link: Installing Nextflow

You’ll need PuTTY on your PC to access the HPC.

You can download PuTTY from here: https://the.earth.li/~sgtatham/putty/latest/w64/putty.exe

Then add the HPC (Lyra) address: lyra.qut.edu.au and then click ‘open’.

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Setup Windows File Explorer to access your HPC home account. Follow the instructions here:

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Copy the Illumina and Nanopore fastq files to their respective workshop directories like so:

Code Block
cd $HOME
cd meta_workshop
cp /work/training/metagenomics/public_data/Illumina*.fastq.gz illumina/fastq
cp /work/training/metagenomics/public_data/Nanopore*.fastq.gz nanopore/fastq

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