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Requirement

  • fasta sequence of custom genome/genome scaffolds

Two-staged process

Run1:

  • The first time running sarek specify the custom fasta file (--fasta mygenome.fa) and add the option to save reference sequence (--save_reference)

    • Sarek will generate a bed, BWAIndex files etc it saved them to the results folder.

    • Copy generated files to reference folder and updated the qutgenomes.config file

Run 2:

  • sarek pipeline is run using generated files in run1

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