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completed

Aligner

GRCh38-local

GRCh38

T2T

star_salmon

  •  completed successfully
  •  completed successfully
  •  completed successfully

star_rsem

  •  did not complete successfully
  •  completed successfully
  •  completed successfully

hisat2

  •  did not complete successfully
  •  
  • did not complete successfully
  •  not tested

SALMON vs. RSEM gene counts:

...

Example - RSEM unique genes:

Code Block
ENSMUSG00000003178 gene_id transcript_id(s)     ENSMUST00000077159   SRR20622172   978.00  889.00SRR20622173  712.00  716.00 SRR20622174 911.00  819.00  1065.00 1214.00 1566.00
ENSMUSG00000029333SRR20622175     SRR20622176    ENSMUST00000031276,ENSMUST00000166484,ENSMUST00000166632,ENSMUST00000168092 SRR20622177    386.00 SRR20622178 256.00  173.00  160.00SRR20622179  358.00  212.00  419.00  508.00SRR20622180
ENSMUSG00000003178  553.00 ENSMUSG00000032134   ENSMUST00000077159   ENSMUST00000034653      12978.00   20889.00   374712.00  445716.00  16911.00   539819.00  291065.00   641214.00   981566.00
ENSMUSG00000035349ENSMUSG00000029333      ENSMUST00000021384ENSMUST00000031276,ENSMUST00000166484,ENSMUST00000166632,ENSMUST00000168092      204386.00  177256.00  103173.00  112160.00  187358.00  137212.00  43419.00   56508.00   67553.00
ENSMUSG00000038194ENSMUSG00000032134      ENSMUST00000127256,ENSMUST00000137350,ENSMUST00000155591ENSMUST00000034653        712.00    1620.8900   15374.2200   10445.8100   1516.00   11539.2400   4429.7200   3264.9200   4098.8200
ENSMUSG00000042402ENSMUSG00000035349      ENSMUST00000045546ENSMUST00000021384      0204.00  177.00  0103.00  112.00  0187.00  137.00  043.00    056.00    067.00
ENSMUSG00000038194     0.00 ENSMUST00000127256,ENSMUST00000137350,ENSMUST00000155591    0.00    07.00 ENSMUSG00000043858   16.89   ENSMUST00000057195,ENSMUST00000107891,ENSMUST0000013686115.22   10.81   15.00  2727.91 196511.9224 668.00  64344.0072  2571.96 75032.9392  786.96  109740.9582
1152.00ENSMUSG00000042402 ENSMUSG00000045946      ENSMUST00000056234ENSMUST00000045546      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000043858 ENSMUSG00000047490     ENSMUST00000057195,ENSMUST00000107891,ENSMUST00000136861  ENSMUST00000050983      02727.0091 1965.92 668.00  0643.00  2571.96 750.93  4786.0096  1097.95  31152.00
ENSMUSG00000045946   1.00    5.00ENSMUST00000056234    1.00    10.00    0.00
ENSMUSG00000048665      ENSMUST00000054470      0.00    0.00    0.00    1.00    0.00    0.00    0.00    0.00
ENSMUSG00000047490   1.00 ENSMUSG00000050347  ENSMUST00000050983    ENSMUST00000052897  0.00    0.00    04.00    03.00    01.00    05.00    01.00    01.00    0.00
ENSMUSG00000048665   0.00 ENSMUSG00000050431      ENSMUST00000051453ENSMUST00000054470      0.00    0.00    0.00    01.00    0.00    0.00    0.00    0.00    01.00
ENSMUSG00000050604ENSMUSG00000050347      ENSMUST00000062454ENSMUST00000052897      60.00    30.00    30.00    30.00    80.00    40.00    50.00    30.00    60.00
ENSMUSG00000050781ENSMUSG00000050431      ENSMUST00000062407ENSMUST00000051453      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000051052ENSMUSG00000050604      ENSMUST00000156004ENSMUST00000062454      06.00    03.00    03.00    03.00    08.00    04.00    05.00    03.00    06.00
ENSMUSG00000050781 ENSMUSG00000052429      ENSMUST00000045325,ENSMUST00000085387,ENSMUST00000107840,ENSMUST00000107842,ENSMUST00000107843,ENSMUST00000125886,ENSMUST00000127531,ENSMUST00000153576 6824.00 4330.00 1250.00 1438.00 6072.00 1652.00 1675.00 2254.00 2492.00
ENSMUSG00000054758      ENSMUST00000067972  ENSMUST00000062407      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000051052   0.00   ENSMUST00000156004  0.00    0.00    0.00
ENSMUSG00000055099      ENSMUST00000068485      0.00    0.00    10.00    30.00    0.00    0.00    0.00
ENSMUSG00000052429   0.00    0ENSMUST00000045325,ENSMUST00000085387,ENSMUST00000107840,ENSMUST00000107842,ENSMUST00000107843,ENSMUST00000125886,ENSMUST00000127531,ENSMUST00000153576 6824.00 ENSMUSG00000055694  4330.00 1250.00 1438.00 6072.00 1652.00 1675.00 2254.00 2492.00
ENSMUSG00000054758    ENSMUST00000046516  ENSMUST00000067972      0.00    0.00    0.00    0.00    0.00    0.00    0.00    10.0900    0.00
ENSMUSG00000056106ENSMUSG00000055099      ENSMUST00000070021ENSMUST00000068485      40.00    10.00    81.9000    3.00    30.00    10.00    50.00    60.00    40.00
ENSMUSG00000056606ENSMUSG00000055694      ENSMUST00000070814ENSMUST00000046516      0.00    0.00    0.00    0.00    0.00    0.00    0.00    01.0009    0.00
ENSMUSG00000056872ENSMUSG00000056106      ENSMUST00000077868ENSMUST00000070021      04.00    01.00    18.0090    13.00    03.00    31.00    05.00    06.00    04.00
ENSMUSG00000057351ENSMUSG00000056606      ENSMUST00000078761ENSMUST00000070814      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000057626ENSMUSG00000056872      ENSMUST00000076465ENSMUST00000077868      20.4900    0.00    21.4900    21.5000    80.6900    23.4900    30.4400    80.9900    70.5800
ENSMUSG00000057832ENSMUSG00000057351      ENSMUST00000080925ENSMUST00000078761      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000057924ENSMUSG00000057626      ENSMUST00000072427ENSMUST00000076465      562.9749    310.00    432.9849    512.0050    668.0069    412.3149    603.0044    528.0099    617.0058
ENSMUSG00000059041ENSMUSG00000057832      ENSMUST00000080251ENSMUST00000080925      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000059101ENSMUSG00000057924      ENSMUST00000079234ENSMUST00000072427      056.0097    031.00    043.0098    051.00    066.00    041.0031    060.00    052.00    061.00
ENSMUSG00000059227ENSMUSG00000059041      ENSMUST00000073059ENSMUST00000080251      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000059448ENSMUSG00000059101      ENSMUST00000079465ENSMUST00000079234      0.00    0.00    20.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000059511ENSMUSG00000059227      ENSMUST00000071632ENSMUST00000073059      0.00    0.00    20.3600    0.00    20.6300    0.00    10.9100    20.5100    0.00
ENSMUSG00000059731ENSMUSG00000059448      ENSMUST00000071975ENSMUST00000079465      0.00    0.00    02.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000059996ENSMUSG00000059511      ENSMUST00000073580ENSMUST00000071632      0.00    0.00    02.0036    0.00    02.0063    0.00    01.0091    02.0051    0.00
ENSMUSG00000060393ENSMUSG00000059731      ENSMUST00000122096ENSMUST00000071975      60.3000    10.7400    30.6900    10.2400    30.7400    10.1900    10.2000    40.8400    20.5800
ENSMUSG00000060559ENSMUSG00000059996      ENSMUST00000074466ENSMUST00000073580      30.00    0.00    160.00   11 0.00    0.00    140.00   14 0.00    300.00   30 0.00
ENSMUSG00000061075ENSMUSG00000060393      ENSMUST00000077247ENSMUST00000122096      06.0030    01.0074    03.0069    01.0024    03.0074    01.0019    01.0020    04.0084    02.0058
ENSMUSG00000061305ENSMUSG00000060559      ENSMUST00000074192ENSMUST00000074466      03.00    0.00    016.00    011.00    0.00    014.00    014.00    030.00   30.00
ENSMUSG00000061075   0.00 ENSMUSG00000061501  ENSMUST00000077247    ENSMUST00000080031  0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000061305      ENSMUST00000074192      0.00
ENSMUSG00000061777    0.00    0.00  ENSMUST00000076936  0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000061501      ENSMUST00000080031      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00
ENSMUSG00000061777      ENSMUST00000076936      0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00    0.00

Run time comparison

GRCm38-local star_salmon

GRCm38-local star_rsem

GRCm38 star_salmon

GRCm38 star_rsem

T2T star_salmon

T2T star rsem

Duration

1h 15m 33s

1h 46m 52s

1h 27m 22s

2h 57m 55s

2h 2m 59s

2h 35m 30s

CPU hours

60.1

139.1

54.4

119.3

139.8

163.1

Wall time

01:27:56

02:58:24

02:03:28

02:36:06

Memory

4,194,304 kb

4,194,304 kb

4,194,304 kb

4,194,304 kb