After trying to change the plotgroups
variable in the Differential Expression section 4 above to one of the other pairwise comparisons, also run through the Functional Annotation analysis for this second comparison.
For example, compare Basal_cells
to Murine_tracheal_epithelial_cell
for the functional annotation analysis.
How does the KEGG pathway and GO term enrichments vary between the two comparisons you’ve just done (i.e. the Basal_cells
to Differentiated_cells
vs Basal_cells
to Murine_tracheal_epithelial_cell
comparison)?