Download Reference microRNA sequences from miRBase
First, let’s download a copy of miRBAse reference sequences, including hairpin and mature microRNA sequences.
microRNA mature sequences:
wget https://mirbase.org/download/mature.fa
Hairpin sequences:
wget https://mirbase.org/download/hairpin.fa
Fetch the genomic coordinated for precursors and mature sequences:
wget https://mirbase.org/download/hsa.gff3
Alternatively, submit the following PBS Pro script to the cluster. Before running the script, create a ‘reference’ folder (i.e., /myteam/data/reference/ ).
#!/bin/bash -l #PBS -N nfsmrnaseq #PBS -l select=1:ncpus=2:mem=4gb #PBS -l walltime=2:00:00 cd $PBS_O_WORKDIR wget https://www.mirbase.org/download/hairpin.fa wget https://www.mirbase.org/download/mature.fa wget https://www.mirbase.org/download/hsa.gff3