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Training and Workshops
2025 - eResearch Hands-On Training Workshops
2024
Introduction to R
2024 - Semester One: Hands-on variant calling and metagenomics analyses using QUT's HPC and Nextflow
2024 - Semester Two: Hands on training workshops - HPC and Gene Expression (RNAseq and small RNAseq) analyses
2024-2 eResearch - Session 2: Intro to HPC
2024-S2 eResearch - Session 3: Introduction to Nextflow
1. Getting started with Nextflow
2. Nextflow pipeline repositories
3. Running pipelines
4. Input specifications
5. Parameters
6. Nextflow caching
7. Nextflow pipeline outputs
8. Where to from now?
2024 eResearch - Session 4: nf-core-RNAseq pipeline
2024-2 eResearch - Session 5: Differential expression analysis using R for RNAseq
2024 eResearch - Session 6 : Small RNAs: A regulatory network of a broad range of biological processes
2024-2 eResearch - Session 7: DE using R for smRNAseq
2023
Start using the HPC
eResearch - single cell RNA-Seq analysis (10x Genomics)
eResearch - writing Nextflow workflows, beginner's guide
2024 eResearch - Session 6 - Hands-on smRNAseq training
Data Download
NextFlow quick start
nf-core/rnaseq - Gene Expression Analysis
nf-core/smrnaseq - Small RNA seq Analysis
nf-core/sarek - variant calling pipeline
nf-core/bactmap: A mapping-based pipeline for creating a phylogeny from bacterial whole genome sequences
nf-epi2me-labs/wf-transcriptomes
nf-eresearch/ONTprocessing - NextFlow pipeline for Oxford Nanopore de novo assembly and ref guided consensus
nf-eresearch/ConsGenome: Nextflow based Genome Assembly, Variant Calling and building a Consensus Genome workflow
nf-eresearch/VirReport - Diagnosis of plant viruses and viroids using small RNA-seq data
nf-core/hic: Analysis of Chromosome Conformation Capture data (Hi-C)
nf-core/ampliseq 16S amplicon analysis pipeline (NextFlow Ampliseq)
nf-core/scrnaseq: A single-cell RNAseq pipeline for 10X genomics data
DESeq2 - Differential expression analysis
PacBio SMRT full length 16S analysis
PacBio SMRT command line Iso-seq analysis
ONT de novo genome assembly
ONT Oxford Nanopore fast5 processing
Variant filtering - screening homozygous recessive mutations
Anacapa - eDNA analysis toolkit
Automatic Assembly For The Fungi (AAFTF) pipeline
Extracting unmapped sequences
primalscheme - designing primer panels for multiplex PCR
Uploading sequence data to SRA
Copy of Copy of Hands-on smRNAseq training
Merging oligonucleotide sequences (local, HPC)
Bisulfite conversion WGBS
Equus caballus Project
Snakemake at QUT
ChIP-Seq
EBV genome integration data analysis
Common Nextflow errors
How to perform KEGG and GO enrichment analysis of non-model species (using R)
Legacy user guides
Nanostring - Nanostring DSP protein assay data QC, exploration, normalisation and analysis.
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