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Aligner

GRCh38-local

GRCh38

T2T

star_salmon

  • completed successfully
  • completed successfully
  • completed successfully

star_rsem

  • did not complete successfully
  • completed successfully
  • completed successfully

hisat2

  • did not complete successfully
  • completed successfully
  • not tested

SALMON vs. RSEM gene counts:

  • No significant changes in feature count values observed for “gene_counts” produced by RSEM or SALMON (see figure below)

  • Most significant changes observed are similar across all samples, hence, differential expression analysis should produce similar results for SALMON and RSEM.

image-20240830-000358.png

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