/
nf-epi2me-labs/wf-transcriptomes

nf-epi2me-labs/wf-transcriptomes

Source:https://github.com/epi2me-labs/wf-transcriptomes

If necessary update the epi2me-labs/wf-transcriptomes container to the latest version:

module load java nextflow pull epi2me-labs/wf-transcriptomes

Create a working directory. For example:

$HOME/epi2me-labs/wf-transcriptomes cd $HOME/epi2me-labs/wf-transcriptomes

Download sample data:

wget -O differential_expression.tar.gz https://ont-exd-int-s3-euwst1-epi2me-labs.s3.amazonaws.com/wf-isoforms/differential_expression.tar.gz && tar -xzvf differential_expression.tar.gz

Prepare a ‘samplesheet.csv’ file for the above data:

Prepare the following ‘launch.pbs PBS Pro script:

Submit the script to the cluster:

Monitor the progress of the job:

Related content

nf-core/scrnaseq: A single-cell RNAseq pipeline for 10X genomics data
nf-core/scrnaseq: A single-cell RNAseq pipeline for 10X genomics data
Read with this
eResearch Paired-end (PE) data sets
eResearch Paired-end (PE) data sets
More like this
5. RNA-seq pipeline
5. RNA-seq pipeline
More like this
Version 3.1.1 - WES variant analysis
Version 3.1.1 - WES variant analysis
More like this
nf-core/hic: Analysis of Chromosome Conformation Capture data (Hi-C)
nf-core/hic: Analysis of Chromosome Conformation Capture data (Hi-C)
More like this
nf-core/chipseq - ChIP-seq peak-calling, QC and differential analysis pipeline.
nf-core/chipseq - ChIP-seq peak-calling, QC and differential analysis pipeline.
More like this